摘要
植物蔗糖转运蛋白是植物中特有的一类跨膜蛋白,负责蔗糖经韧皮部从源到库的运输以及库组织的蔗糖供给。为了解析单子叶特异的玉米蔗糖转运蛋白基因ZmSUT3的生物学功能,本研究利用RT-PCR方法,从玉米自交系‘吉853’的基因组中克隆得到蔗糖转运蛋白ZmSUT3基因,命名为ZmSUT3J。测序结果表明:开放阅读框(ORF)长度为1527 bp,编码508个氨基酸的蛋白质即ZmSUT3,分子量为53.51 kD。利用生物信息学的方法分析ZmSUT3蛋白属于疏水的MFS蛋白超家族,含有多个磷酸化位点,主要定位在质膜和叶绿体类囊体膜上,具有12个弯曲交叉的螺旋结构,属于跨膜蛋白。系统进化树分析表明,ZmSUT3蛋白属于SUT3亚族,与高粱的Sb SUT3亲缘关系较近。启动子区域生物信息学分析结果显示,含有多个组织特异性调控元件,还有激素调节及胁迫诱导相关顺式作用元件,推测ZmSUT3在库组织中发挥作用,参与激素相关胁迫应答反应。为验证ZmSUT3J基因的生物学功能,本研究构建了植物表达载体,为研究ZmSUT3在玉米中的功能奠定了基础。
Plant sucrose transporters are plant-specific transmembrane proteins in plants, which are responsible for transmembrane translocation of sucrose from source to reservoir through phloem and sucrose supply to reservoir tissue. To study the biological functions of monocot-specific sucrose transporter gene Zm SUT3, reverse transcriptionPCR(RT-PCR) was employed in this study, to clone the Zm SUT3 gene from Zea mays inbred line ’Ji853’, termed Zm SUT3 J. The sequence analysis indicated that ZmSUT3 J had an open reading frame(ORF) of 1 527 bp and encoded a protein named ZmSUT3 of 508 amino acids residues. By the help of bioinformatics methods, the encoded protein was analyzed for physicochemical properties, subcellular localization prediction, and protein structure prediction. The results showed that Zm SUT3 protein belonged to the hydrophobic and MFS superfamily,which had a number of phosphorylation sites, and located in the plasma membrane and chloroplast thylakoid membrane. ZmSUT3 J protein had 12 transmembrane helix domains and belonged to transmembrane proteins. The phylogenetic tree showed that Zm SUT3 J belonged to the SUT3 subfamily, and it was really close to SbSUT3 from sorghum. Bioinformatics analysis of ZmSUT3 promoter showed that there were several tissue-specific regulatory sites, and many cis-regulating elements involved in plant hormone regulation and stress-inducible regulation. It was deduced that ZmSUT3 might play an important role in sink organs, and involve in stress regulatory response.To elucidate the biological functions of ZmSUT3 J, transgenic technology was used to validate its functions. The results built the foundation for ZmSUT3 functional analysis in maize.
作者
李楠
朱旭
柳青
郭嘉
孙传波
李传龙
贺红利
贺红霞
Li Nan;Zhu Xu;Liu Qing;Guo Jia;Sun Chuanbo;Li Chuanlong;He Hongli;He Hongxia(ilin Province Key Laboratory of Agricultural Biotechnology,Institute of Agricultural Biotechmology,JAAS,Changchun,130033;College of Life Sciences,Jilin Normal University,Siping.136000)
出处
《分子植物育种》
CAS
北大核心
2021年第7期2111-2122,共12页
Molecular Plant Breeding
基金
吉林省科技发展计划项目(20170101023JC)
吉林省农业科技创新工程项目(CXGC2017ZY028)共同资助。
