摘要
采用80%(体积分数,下同)乙醇超声提取澳洲坚果青皮,依次用石油醚、氯仿、乙酸乙酯、正丁醇4种不同极性溶剂对其提取物进行分步萃取,剩余为水溶解物,分别得到80%乙醇提取物、石油醚提取物、氯仿提取物、乙酸乙酯提取物、正丁醇提取物与水溶解物6种溶剂提取物,测定其总酚、黄酮、多糖等主要功能成分质量分数与抗氧化活性,并分析其相关性。结果表明:总酚和黄酮质量分数在澳洲坚果青皮乙酸乙酯提取物中均最高,分别为(40.36±0.48)%与(41.68±0.93)%,多糖质量分数在正丁醇提取物中最高,为(22.08±2.09)%。澳洲坚果青皮不同极性溶剂分步提取物具有较强的2,2’-联氮-双-3-乙基苯并噻唑啉-6-磺酸(2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid),ABTS)阳离子自由基清除能力与较高的还原力,对1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-picrylhydrazyl,DPPH)自由基与超氧阴离子自由基拥有一定的清除能力。其中,乙酸乙酯提取物对DPPH自由基、ABTS阳离子自由基的清除能力最强,还原力最高,其半抑制浓度(half maximal inhibitory concentration,IC_(50))分别为0.67、0.05、0.09 mg/mL。正丁醇提取物对超氧阴离子自由基的清除能力最强,其IC_(50)为0.08 mg/mL,相同质量浓度下优于芦丁。通过对各指标进行皮尔逊相关性分析得出,澳洲坚果青皮不同极性溶剂分步提取物对DPPH自由基清除能力、ABTS阳离子自由基清除能力与还原力均与其总酚、黄酮、多糖质量分数呈极显著正相关(P<0.01),对超氧阴离子自由基的清除能力与其多糖质量分数呈极显著正相关(P<0.01)。应用多元回归分析建立了澳洲坚果青皮不同极性溶剂分步提取物DPPH自由基清除能力(Y_(1))、超氧阴离子自由基清除能力(Y_(2))、ABTS阳离子自由基清除清除能力(Y_(3))、还原力(Y_(4))与其功能成分(总酚(X_1)、黄酮(X_(2))、多糖(X_(3))与皂苷及其他成分(X_4))质量分数之间的线性回归方程,分别为Y_(1)=7.634 4-0.071 0X_(1)+0.170 2X_(2)+0.227 6X_(3)-0.013 3X_(4)、Y_(2)=29.024 5-0.405 0X_(1)+0.320 0X_(2)+0.597 2X_(3)、Y_(3)=40.305 6+0.188 8X_(1)+0.030 4X_(4)与Y_(4)=0.298 2+0.004 5X_(2)+0.006 0X_(3)。结论:研究为开发利用澳洲坚果青皮资源提供一定的技术依据。
The contents of total phenols,total flavonoids and polysaccharides and the in vitro antioxidant activities of petroleum ether(PE),chloroform(CE),ethyl acetate(EAE),n-butanol(NBE)and water soluble extracts obtained from successive fractionation of the 80%ethanol extract(EE)of macadamia green husk were evaluated and the correlation between the bioactive components and the antioxidant activities was analyzed.The results showed that the contents of total phenols and flavonoids in EAE were the highest among all samples,(40.36±0.48)%and(41.68±0.93)%,respectively,and the content of polysaccharides in NBE was the highest,(22.08±2.09)%.EE and fractions thereof all had strong scavenging capacity against 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)(ABTS)cation radicals and reducing power,and also had certain scavenging capacity against 1,1-diphenyl-2-picrylhydrazyl(DPPH)and superoxide anion radicals.EAE had the strongest scavenging capacity against DPPH and ABTS cation radicals and the highest reducing power with half maximal inhibitory concentration(IC50)of 0.67,0.05 and 0.09 mg/mL,respectively.NBE had the strongest scavenging capacity against superoxide anion radicals with IC50 of 0.08 mg/mL,which was better than rutin at the same concentration.Pearson correlation analysis showed that for all samples,the scavenging effect on DPPH and ABTS cation radicals and reducing power were significantly positively correlated with the contents of total phenols,flavonoids and polysaccharides(P<0.01),and the scavenging effect on superoxide anion radicals was significantly positively correlated with the content of polysaccharides(P<0.01).The linear regression equations between antioxidant properties and the contents of total phenols(X_(1)),flavonoids(X_(2)),polysaccharides(X_(3))and Saponins and other components(X4)were established as follows:Y_(1)=7.6344-0.0710 X_(1)+0.1702 X_(2)+0.2276 X_(3)-0.0133 X_(4) for DPPH scavenging capacity,Y_(2)=29.0245-0.4050 X_(1)+0.3200 X_(2)+0.5972 X_(3) for superoxide anion radical scavenging capacity,Y_(3)=40.3056+0.1888 X_(1)+0.0304 X_(4) for ABTS cation radical scavenging capacity,and Y_(4)=0.2982+0.0045 X_(2)+0.0060 X_(3) for reducing power,respectively.Conclusion:This study provides a certain technical basis for the development and utilization of macadamia green husk.
作者
郭刚军
胡小静
付镓榕
马尚玄
徐荣
黄克昌
彭志东
贺熙勇
邹建云
GUO Gangjun;HU Xiaojing;FU Jiarong;MA Shangxuan;XU Rong;HUANG Kechang;PENG Zhidong;HE Xiyong;ZOU Jianyun(Yunnan Institute of Tropical Crops,Jinghong 666100,China;School of Chemistry and Engineering,Wenshan University,Wenshan 663000,China)
出处
《食品科学》
EI
CAS
CSCD
北大核心
2021年第7期74-82,共9页
Food Science
基金
云南省热带作物科技创新体系建设专项(RF2020-14,RF2019-11,RF2018-10)
云南省热带作物科学研究所青年成长基金项目(QNCZ2020-5)。
关键词
澳洲坚果青皮
不同极性溶剂
分步提取物
功能成分
抗氧化活性
相关性
macadamia green husk
different polar solvents
successive fractionation
functional components
antioxidant activity
correlation
