摘要
本文在单因素试验(乙醇体积分数、提取时间、离心时间)的基础上进行了正交试验,以优化新鲜烟叶可溶性蛋白的提取工艺。结果表明,烟叶FⅡ蛋白提取的最佳工艺条件为乙醇体积分数70%、提取时间2.0 h、离心时间15 min。在此条件下,FⅡ蛋白提取率为75.96%。试验结果为新鲜烟叶可溶性蛋白的提取及利用提供了参考。
In this paper,on the basis of single factor experiment(ethanol volume fraction,extraction time,centrifugal time),an orthogonal experiment was carried out to optimize the extraction process of soluble protein in fresh tobacco leaves.The results showed that the optimal process conditions for extracting tobacco FⅡprotein was ethanol volume fraction 70%,extraction time 2.0 h,and centrifugal time 15 min.Under these conditions,the extraction rate of FⅡprotein was 75.96%.The test results provide references for the extraction and utilization of soluble protein in fresh tobacco leaves.
作者
杨会
张元梅
郭翠
王丹
罗逸
谭艾娟
YANG Hui;ZHANG Yuanmei;GUO Cui;WANG Dan;LUO Yi;TAN Aijuan(College of Life Science,Guizhou University,Guiyang Guizhou 563200)
出处
《现代农业科技》
2021年第9期242-244,共3页
Modern Agricultural Science and Technology
关键词
烟叶
FⅡ蛋白
提取工艺
单因素试验
正交试验
tobacco leaf
FⅡprotein
extraction process
single factor experiment
orthogonal experiment
