摘要
随着烟草工业的发展,对烟草样品进行快速、准确和高灵敏度地分析检测已经成为烟草化学发展的必然趋势和产品研发的现实需求。本工作利用超声雾化萃取大气压光电离质谱(EAPPI-MS)技术,无需样品预处理和色谱分离,对1R5F、3R4F、都宝、七星和王冠5种卷烟烟丝中的化学成分进行直接、快速分析,鉴别出醇、酮、酸、酯、醛、酚、生物碱、氨基酸和萜类等46种物质。与传统的电喷雾电离液相色谱-质谱(LC-ESI-MS)法相比,EAPPI-MS具有无极性歧视、受基质效应影响小等优点,可检测出更加丰富的化学成分。经实验优化后,选取二氯甲烷-甲醇溶液(2∶3,V/V)为萃取溶剂,分别分析了5种卷烟烟丝中的主要生物碱、有机酸和酚类的相对含量,并研究了相对含量的差异与烟草品质之间的相关性。根据获取的EAPPI-MS质谱图,借助主成分分析法(PCA)对5种不同种类的卷烟烟丝实现了快速区分。该测试过程简单快速、定性定量准确,可用于烟叶和各种烟草制品化学成分的快速批量化测定。
With the development of the tobacco industry,rapid,accurate,and high sensitive analysis of tobacco samples has become an inevitable trend and a practical need for the development of the tobacco industry.In this paper,extractive atmospheric pressure photoionization mass spectrometry(EAPPI-MS)was used to directly and rapidly analyze the chemical components in 5 kinds of tobacco products including 1R5F,3R4F,Derby,Seven Stars,and Wang Guan.This method did not require sample pretreatment and chromatography separation.The whole detection took only one minute for one sample.46 kinds of chemical components in tobacco including alcohols,ketones,acids,esters,aldehydes,phenols,alkaloids,amino acids,and terpenoids were identified.Compared with the traditional electrospray ionization(ESI),EAPPI has the advantages of nopolar discrimination and higher tolerance to the complex matrix.More than this,more chemical components can be detected by EAPPI-MS than liquid chromatography-electrospray ionization mass spectrometry(LC-ESI-MS).After comparing the extraction capacity of extraction solvents with different polarities,dichloromethane-methanol(2∶3,V/V)showed a higher extraction effect,which was selected as the optimized extraction solvent.Under optimized parameters,the relative contents of the main alkaloids,organic acids,and phenols in the 5 kinds of tobacco products were analyzed using EAPPI-MS,respectively.Meanwhile,the correlation between the relative contents of tobacco chemicals and tobacco quality was discussed.Overall,different types of tobacco products were unique because of their unique chemical contents.Among these three commercial tobaccos,Derby and Seven Stars were more flavored than Wang Guan.According to the obtained mass spectra of m/z 50-350 using EAPPI-MS,the 5 kinds of tobacco products could be rapidly distinguished using principal component analysis(PCA).EAPPI-MS provides a simple,rapid,and accurate qualitative and quantitative analysis for tobacco chemicals.This method can be used for rapid batch test of chemical components in tobacco leaves and various tobacco products.
作者
赵婉
王健
戚可可
胡永华
吴刘天
刘成园
潘洋
ZHAO Wan;WANG Jian;QI Ke-ke;HU Yong-hua;WU Liu-tian;LIU Cheng-yuan;PAN Yang(National Synchrotron Radiation Laboratory,University of Science and Technology of China,Hefei 230029,China;Center of Technology,China Tobacco Anhui Industrial Co.,Lt.,Hefei 230088,China)
出处
《质谱学报》
EI
CAS
CSCD
北大核心
2021年第3期271-284,I0003,共15页
Journal of Chinese Mass Spectrometry Society
基金
安徽中烟工业有限责任公司科技计划项目(2017044)资助。
关键词
萃取
大气压光电离质谱
烟草
化学成分
extraction
atmospheric pressure photoionization mass spectrometry(APPI-MS)
tobacco
chemical components
