摘要
为探究不同富营养化水体沉积物中细菌群落结构的差异及驱动因素,以白洋淀表层沉积物细菌为研究对象,通过高通量测序方法,分析了2种富营养水平下细菌群落结构组成、多样性以及与环境因子的相关性。结果表明,细菌群落多样性随富营养化水平升高而下降;重度和中度富营养化水平下的细菌群落结构有差异,重度富营养化水平下绿弯菌门丰度显著提高。总体而言,不同污染程度水体沉积物细菌群落结构在门水平上没有明显差异,沉积物中的优势菌门依次为变形菌门(31%~43%)、绿弯菌门(10%~21%)和拟杆菌门(9%~20%)。微生物群落与环境因子相关性热图表明,上覆水TP和NH_(4)^(+)-N、底泥TP和NO_(3)^(-)-N是白洋淀水体底泥细菌群落多样性的主要影响因素。白洋淀不同富营养化水体沉积物细菌多样性存在差异,磷含量是细菌群落结构的制约因素。
The purpose of this study was to explore the differences and driving factors of bacterial community structure in sediments of different eutrophic water bodies in Baiyangdian Lake.The composition and diversity of bacterial community structure under two eutrophication levels in surface sediment,as well as their correlation with environmental factors,were studied through high-throughput sequencing.The results showed that the diversity of bacterial communities decreased with the increase of the eutrophication level.The bacterial community structure was different under the severe and moderate eutrophication levels,and the abundance of Chloroflexi increased significantly under the severe eutrophication level.However,there was no significant difference in the bacterial community structure in Phylum level with different eutrophication in generally.The predominant phylum in the sediments were Proteobacteria(31%~43%),Chloroflexi(10%~21%)and Bacteroidetes(9%~20%),respectively.The heatmap of the correlation between microbial communities and environmental factors revealed that TP and NH_(4)^(+)-N in surface water,TP and NO_(3)^(-)-N in sediments were the main influencing factors of bacteria community diversity in the Baiyangdian Lake.It can be concluded that the diversity of bacteria in sediment was dissimilar in different eutrophic water,and the phosphorus content was identified as the limiting factor of bacterial community structure in Baiyangdian Lake.
作者
于妍
王悦悦
方杜贤
任洁
王颖
YU Yan;WANG Yueyue;FANG Duxian;REN Jie;WANG Ying(School of Chemical and Environmental Engineering,China University of Mining and Technology(Beijing),Beijing 100083,China;Key Laboratory of Water and Sand Science,Ministry of Education,College of Environmental Sciences,Beijing Normal University,Beijing 100875,China)
出处
《环境工程学报》
CAS
CSCD
北大核心
2021年第3期1121-1130,共10页
Chinese Journal of Environmental Engineering
基金
国家水体污染控制与治理科技重大专项课题(2018ZX07110004)
北京市大学生科学研究与创业行动计划项目资助(C201903746)。
关键词
白洋淀
富营养化水体
沉积物
微生物群落结构
环境因子
baiyangdian Lake
eutrophication bodies
sediments
bacterial community structure
environmental factors
