摘要
目的观察microRNA-21(miR-21)敲除对耐伊马替尼的人慢性髓性白血病细胞株K562/G01细胞在增殖、药物敏感性等方面的影响,初步探讨miR-21影响K562/G01细胞伊马替尼敏感性的可能机制。方法运用CRISPR/Cas9技术敲除K562/G01细胞的miR-21,经PCR筛选、Sanger测序鉴定和实时定量PCR检测获得miR-21敲除的单细胞克隆。扩增培养后,采用MTT法、细胞克隆形成实验检测miR-21敲除对K562/G01细胞增殖的影响。使用伊马替尼处理细胞后,用MTT法和AnnexinⅤ-APC/7-AAD双染流式细胞检测法观察敲除miR-21后K562/G01细胞对伊马替尼的敏感性的变化。Western blot法检测miR-21敲除前后K562/G01细胞PTEN、AKT、p-AKT、PI3K、p-PI3K、P210^(BCR-ABL)、p-P210^(BCR-ABL)蛋白表达量的变化。结果成功构建了3个miR-21敲除的K562/G01单细胞克隆,CRISPR/Cas9介导的突变效率为7.12%~8.11%。miR-21敲除使K562/G01细胞的增殖受抑,野生型和1#、2#、6#单细胞克隆的克隆形成率依次为(57.67±8.25)%、(26.94±5.36)%、(7.17±2.11)%、(31.50±3.65)%,差异有统计学意义(P<0.05)。miR-21敲除使K562/G01细胞对伊马替尼的敏感性增加,野生型和1#、2#、6#单细胞克隆对伊马替尼的IC_(50)值分别为(21.92±1.36)µmol/ml、(3.98±0.39)µmol/ml、(5.38±1.01)µmol/ml、(9.24±1.36)µmol/ml,差异有统计学意义(P<0.05)。miR-21敲除后,其靶基因PTEN的蛋白表达水平未见明显变化,但PI3K、AKT信号分子的活化受到抑制,并且P210^(BCR-ABL)、p-P210^(BCR-ABL)蛋白表达也下调。结论miR-21敲除抑制K562/G01细胞增殖,提高其对伊马替尼的敏感性,这可能是通过抑制PI3K/AKT信号通路和BCR-ABL表达实现的。
Objective To observe the effects of miR-21 knockout on proliferation and drug resistance in K562/G01 cells,and to preliminarily explore the mechanism of imatinib sensitivity by knocking out miR-21 in K562/G01 cells.Methods Using CRISPR/Cas9 to knock out the miR-21 gene in K562/G01 cells,and single-cell-derived clones of miR-21 knockout were obtained by genomic DNA PCR screening,Sanger sequencing,and real-time PCR.We used MTT and cell colony formation assays to assess the cell proliferation,and determined imatinib sensitivity by MTT assay and Annexin-Ⅴ-APC/7-AAD double staining flow cytometry.Using western blot,we examined the potential mechanisms affecting imatinib sensitivity by knocking out miR-21 in K562/G01 cells.Results Three miR-21 knockout K562/G01 single-cell-derived clones were successfully constructed.The mutation efficiency mediated by CRISPR/Cas9 was 7.12%-8.11%.MiR-21 knockout inhibited the proliferation of K562/G01 cells;the clone formation rates of WT and 1#,2#,6#K562/G01 single-cell clones were(57.67±8.25)%,(26.94±5.36)%,(7.17±2.11)%,(31.50±3.65)%,respectively.MiR-21 knockout increased the sensitivity of K562/G01 cells to imatinib,IC_(50)of imatinib in WT,and 1#,2#,6#K562/G01 single-cell clones were(21.92±1.36)µmol/ml,(3.98±0.39)µmol/ml,(5.38±1.01)µmol/ml,(9.24±1.36)µmol/ml.After the knockout of miR-21,the activation of PI3K/Akt signaling molecules was inhibited,while the expression of P210^(BCR-ABL)and p-P210^(BCR-ABL)was downregulated;however,the expression of PTEN was not affected.Conclusion The knockout of miR-21 can suppress cell proliferation and improve sensitivity to imatinib in K562/G01 cells,which may be achieved by inhibiting the PI3K/AKT signaling pathway and BCR-ABL expression.
作者
张雲
王凌燕
李佳蒸
江佩芳
胡建达
陈步远
Zhang Yun;Wang Lingyan;Li Jiazheng;Jiang Peifang;Hu Jianda;Chen Buyuan(Fujian Medical University Union Hospital,Fujian Institute of Hematology,Fujian Provincial Key Laboratory of Hematology,Fuzhou 350001,China)
出处
《中华血液学杂志》
CAS
CSCD
北大核心
2021年第3期243-249,共7页
Chinese Journal of Hematology
基金
福建省自然科学基金(2017J05129)
国家自然科学基金青年科学基金(81600137)。
