摘要
土壤真菌群落多样性和菌群关系是维持生态系统的多样性及稳定性的关键。本文以粗枝云杉人工林和天然林为研究对象,利用高通量测序技术和生物信息学分析方法,研究了云杉根际和非根际土壤真菌群落组成、多样性及菌群网络关系。结果表明:从群落组成上看,人工林中相对丰度最高的科是丝盖伞科,而天然林中是蜡壳耳科,两处林型下占比最高的属均为丝盖伞属。群落的β多样性在两处林型的根际、非根际下存在显著差异。环境变量与真菌类群的相对丰度和α多样性相关关系不显著,而草本覆盖度、土壤含水率、总有机碳和植被丰富度是群落β多样性的主要影响因素。网络分析显示,天然林土壤真菌菌群之间以负相关关系为主,表明天然林土壤中菌群之间主要存在竞争作用。比较两处林型下的根际、非根际土壤真菌菌群关系发现,非根际区域菌群之间负相关性均较高,表明非根际土壤中菌群的种间竞争作用可能要强于根际土壤。结合差异丰度分析,两处林型下根际和非根际之间存在显著差异的物种中仅有蜡壳耳科为真菌网络中共有的关键菌群,表明人工林和天然林土壤真菌群落结构中差异种群的变化可能对其群落稳定性影响较小。
The diversity and interactions of soil fungal community are the key to maintain the diversity and stability of ecosystem.In this study,we examined the structure,diversity and co-occurrence networks of fungal community in rhizosphere and non-rhizosphere soils of planted and natural Picea asperata forests using high-throughput sequencing technique and bioinformatic methods.The results showed that Inocybaceae and Sebacinaceae were dominant family in soils of planted and natural forests,respectively.At the genus level,Inocybe was dominant one in soils of planted and natural forests.There were significant differences inβ-diversity of fungal communities between rhizosphere and non-rhizosphere soils in both planted and natural forests.There were no significant correlations between environmental variables and the relative abundance andα-diversity of fungal communities.Herb layer coverage,soil water content,total organic carbon concentration,and plant species richness played important roles in explaining the variations ofβ-diversity of fungal communities.Results of the network analysis showed that the negative correlations were dominant among soil fungal communities in natural forest,suggesting that the competition of different groups in natural forest.Moreover,there were more negative correlations in non-rhizosphere soils than in rhizosphere soils,which indicated that fungal communities in non-rhizosphere soils comprised more competitive network structure than in the rhizosphere soils.Biomarker species were identified based on differential abundance analysis.Sebacinaceae was the single shared keystone species in the fungal network which had significant differences among rhizosphere and non-rhizosphere soils of planted and natural forests.Therefore,it is suggested that the variation of differential species in the soil fungal communities between the planted and natural forest might had limited influence on the stability of the community of planted and natural forests.
作者
刘艳娇
樊丹丹
李香真
赵文强
寇涌苹
LIU Yan-jiao;FAN Dan-dan;LI Xiang-zhen;ZHAO Wen-qiang;KOU Yong-ping(Engineering Research Center of Soil Remediation of Fujian Province University,College of Resources and Environment,Fujian Agriculture and Forestry University,Fuzhou 350002,China;Chengdu Institute of Biology,Chinese Academy of Sciences,Chengdu 610041,China)
出处
《应用生态学报》
CAS
CSCD
北大核心
2021年第4期1441-1451,共11页
Chinese Journal of Applied Ecology
基金
中国科学院西部之光项目(Y8C2041100)
四川省科技厅应用基础研究重点项目(2019YJ0010)资助。
关键词
亚高山森林
粗枝云杉
真菌群落
高通量测序
分子生态网络
subalpine forest
Picea asperata
fungal community
high-throughput sequencing
molecular ecological network
