摘要
为揭示意大利蜜蜂哺育蜂上颚腺中蜂王浆合成和分泌相关的分子机制,通过组建人工蜂群收集3日龄工蜂(3d)、10日龄哺育蜂(10dN)、10日龄采集蜂(10dF)、21日龄哺育蜂(21dN)和21日龄采集蜂(21dF),利用高效液相色谱技术检测5种不同职能工蜂上颚腺中10-羟基-2-癸烯酸(10-hydroxy-2(E)-decenoic acid,10-HDA)的含量,并在转录组学水平全面分析上颚腺中调控蜂王浆合成和分泌密切相关的基因。结果表明:工蜂上颚腺中10-HDA含量随日龄的增加呈逐渐增加趋势,其中21dF和21dN上颚腺中10-HDA的含量均显著高于3d(P<0.05),但同日龄采集蜂上颚腺中10-HDA的含量高于相同日龄哺育蜂;RNA-seq分析发现,46个差异基因在10dN上颚腺中的表达量显著高于3d、10dF和21dF,同时在21dN上颚腺中的表达量也显著高于21dF;GO和KEGG富集分析显示这些差异基因主要富集在脂肪酸代谢、能量代谢等方面,暗示哺育蜂的上颚腺可能通过增加脂肪酸代谢和能量代谢等促进蜂王浆中相关脂肪酸的合成。综上,在排除日龄影响下,本研究测定了不同职能工蜂上颚腺中10-HDA的变化趋势,同时筛选了46个与哺育蜂上颚腺中蜂王浆合成和分泌密切相关的基因,为深入阐明其分子机制提供基础,也为研究膜翅目昆虫上颚腺的功能提供新视角。
In order to reveal the molecular mechanism related to the synthesis and secretion of royal jelly in the mandibular glands(MGs)of nurses,specimens(3-day-old worker bees(3 d),10-day-old nurses(10 dN),10-day-old foragers(10 dF),21-day-old nurses(21 dN)and 21-day-old foragers(21 dF))were collected from an established bee colony.The content of 10-hydroxy-2(E)-decenoic acid(10-HDA)was detected in the MGs of worker bees with five different functions by high-performance liquid chromatography(HPLC)and genes closely related to the synthesis and secretion of royal jelly were analyzed comprehensively in the MGs at the transcriptional level.The results showed that:The content of 10-HDA in the MGs of worker bees increased gradually with the increase of age.The content of 10-HDA in the MGs of 21 dN/F were both significantly higher than that of 3 d(P<0.05),but 10-HDA content of foragers was higher than that of nurses with corresponding age;RNA-seq revealed that the expression of 46 differentially expressed genes(DEGs)in the MGs of 10 dN was significantly higher than those of 3 d,10 dF and 21 dF,and also those DEGs in the MGs of 21 dN was significantly higher than that of 21 dF;GO and KEGG enrichment analysis showed that upregulated DEGs were mainly enriched in fatty acid metabolism and energy metabolism,suggesting that the MGs of nurses might increase fatty acid metabolism and energy metabolism to stimulate the synthesis of related fatty acids during the production of royal jelly.In summary,the variation trend of 10-HDA in MGs of worker bees with different performances was determined without the influence of age,and 46 DEGs associated with the secretion of royal jelly were identified,which provide a great deal of valuable information for further elucidating the molecular mechanism underlying the production of royal jelly,and also will provide a new perspective for studying the function of MGs in hymenoptera insects.
作者
高艳
杨振慧
李秋方
朱雅楠
梁立强
苏松坤
聂红毅
GAO Yan;YANG Zhenhui;LI Qiufang;ZHU Yanan;LIANG Liqiang;SU Songkun;NIE Hongyi(College of Animal Sciences(College of Bee Science),Fujian Agriculture and Forestry University,Fuzhou 350002,China;Jiangxi Apiculture Research Institute,Fuzhou 350002,China;Center for Genomics and Biotechnology,Fujian Agriculture and Forestry University,Nanchang 330052,China)
出处
《中国农业大学学报》
CAS
CSCD
北大核心
2021年第4期93-103,共11页
Journal of China Agricultural University
基金
国家现代农业产业技术体系(蜜蜂)项目(CARS-44-KXJ4)
国家自然科学基金面上项目(31772684)
福建省自然科学基金项目(2018J05043)
福建省省属高校科研项目(JK2017014)。
