摘要
以福建省惠安赤湖国有防护林场3个不同连栽代数(第一代first rotation plantation,FCP;第二代second rotation plantation,SCP;第三代third rotation plantation,TCP)木麻黄根际土壤为研究对象,运用高效液相色谱技术(HPLC)研究连栽木麻黄根系分泌物-酚酸种类及含量变化特征,并模拟根际土壤中各酚酸配比,探究酚酸类物质对木麻黄根际土壤关键微生物生长的影响。结果显示:连栽木麻黄土壤中检测到香草酸、阿魏酸、没食子酸、对香豆酸和水杨酸5种酚酸类物质,平均配比为32∶6∶19∶5∶8,其总含量随着连栽代数增加而上升;其中,香草酸、没食子酸、对香豆酸和水杨酸在连栽土壤SCP、TCP中有所累积,但阿魏酸含量随着连栽代数增加而下降,差异不显著(P>0.05);模拟木麻黄根际土壤中平均配比的混合酚酸显著促进了病原菌-尖孢镰刀菌生长,其中水杨酸的促进作用最强;相反,该混合酚酸能抑制有益拮抗菌-解淀粉芽孢杆菌的生长,其中水杨酸的抑制作用最强。由此可知,木麻黄根系分泌物-酚酸对根际土壤微生物群落具有特异选择性,引起根际微生物群落结构失衡,进而导致连栽障碍问题。研究结果为缓解木麻黄人工林生产经营中存在的连栽障碍问题提供理论参考。
We examined the species and content of phenolic acids in rhizosphere soil of replanted Casuarina equisetifolia plantations(including first rotation plantation(FCP),second rotation plantation(SCP)and third rotation plantation(TCP))in Fujian Huian Chihu State-owned Shelter Forest Farm by high performance liquid chromatography(HPLC).The ratio of phenolic acids in rhizosphere soil was simulated to explore its effect on the growth of key soil microbes.The results showed that five kinds of phenolic acids were detected in the root exudates of C.equisetifolia,including vanillic acid,ferulic acid,gallic acid,cumaric acid,and salicylic acid,with an average ratio of 32∶6∶19∶5∶8.The total content of phenolic acids increased with increasing rotation generations.Among the five phenolic compounds,vanillic acid,gallic acid,cumaric acid,and salicylic acid accumulated in SCP and TCP,while the content of ferulic acid showed a decreasing trend with increasing rotation generations(P>0.05).The phenolic acids mixture at their ratios explored in the C.equisetifolia soil significantly accelerated mycelial growth of Fusarium oxysporum,but inhibited Bacillus amyloliquefacien.Salicylic acid had the strongest positive or negative effects on the growth of pathogenic or beneficial microorganisms.Therefore,the mixture of phenolic compounds played a role in affecting the growth of soil microorganisms,resulting in the structure imbalance of rhizosphere microbial community,and consequently leading to consecutive planting obstacles.Our results provide a theoretical reference for alleviating consecutive planting obstacles in the production and management of C.equisetifolia plantations.
作者
周柳婷
罗扬
李建鹃
赵艳琳
白莹
张晨
陈军
林文雄
吴则焰
ZHOU Liu-ting;LUO Yang;LI Jian-juan;ZHAO Yan-lin;BAI Ying;ZHANG Chen;CHEN Jun;LIN Wen-xiong;WU Ze-yan(Life Sci-ences College of Fujian Agriculture and Forestry University,Fuzhou 350002,China;ForestryCollege of Fujian Agriculture and Forestry University,Fuzhou 350002,China;Key Laboratory of Crop Ecology and Molecular Physiology,Fujian Agriculture and Forestry University,Fuzhou350002,China;Fujian Provincial Key Laboratory of Agro-ecological Processing and Safety Moni-toring,School of Life Sciences,Fujian Agriculture and Forestry University,Fuzhou 350002,China)
出处
《生态学杂志》
CAS
CSCD
北大核心
2021年第4期1021-1028,共8页
Chinese Journal of Ecology
基金
国家自然科学基金项目(31500443)
福建省自然科学基金项目(2018J01617)
福建农林大学杰青项目(XJQ201718)
福建农林大学科技创新专项项目(CXZX2019051G)资助。
关键词
连栽障碍
木麻黄
酚酸类物质
高效液相色技术
consecutive planting obstacle
Casuarina equisetifolia
phenolic compounds
high performance liquid chromatography(HPLC)
