摘要
目的:明确闹羊花的挥发性成分并比较不同花期样品的挥发性成分差异。方法:采用气相色谱-离子迁移谱法(GC-IMS)对5个不同花期(花蕾期、初开期、半开期、盛开期、盛开末期)闹羊花样品的挥发性成分进行检测;利用GC-IMS谱图结合聚类分析、主成分分析(PCA)和正交偏最小二乘法-判别分析(OPLS-DA)比较不同花期样品的挥发性成分异同。结果:GC-IMS检测出了不同花期闹羊花中的70种挥发性成分,其中67种为共有成分,定性确定了47种,以醇类、酯类、醛类为主。香芹醇是盛开末期样品特有成分;α-松油醇在初开期样品中的含量最高,盛开期、盛开末期样品中则未检出。活性成分(甲基庚烯酮,壬醛,α-松油醇,桉叶油醇,氧化芳樟醇,1-辛烯-3-醇,反式-3-己烯-1-醇)相对质量分数之和在花期中呈下降趋势。GCIMS图谱显示不同花期样品有各自的特征峰区域,也有共同区域。聚类分析,PCA及OPLS-DA结果均显示,不同花期样品区分明显;通过OPLS-DA筛选获得19种区分不同花期样品的差异成分,包括γ-丁内酯、桉叶油醇、乙酸乙酯等。结论:不同花期闹羊花样品可明显区分且其挥发性成分中的活性物质随花开放的程度逐步散失,可为闹羊花的物质基础完善及不同花期研究提供参考。
Objective: The volatile components of Rhododendri Mollis Flos were determined and the differences of volatile components at different flowering stages were compared and analyzed. Method: Gas chromatography-ion mobility spectrometry(GC-IMS) was used to detect the volatile components in Rhododendri Mollis Flos at different flowering stages(bud stage,initial flowering stage,half-flowering stage,blooming stage and late blooming stage). GC-IMS spectra combined with cluster analysis,principal component analysis(PCA)and orthogonal partial least squares-discriminant analysis(OPLS-DA)were used to compare the differences and similarities of volatile components in different flowering stages. Result: A total of 70 volatile components in Rhododendri Mollis Flos at different flowering stages were detected,among which 67 were common components,and 47 were identified qualitatively,mainly alcohols,esters and aldehydes. Carveol was a special component at the late blooming stage. The content of alpha-terpineol is the highest at the initial flowering stage,but not at the blooming stage and late blooming stage. The relative contents of the active ingredients [6-methyl-5-hepten-2-one,nonanal,alpha-terpineol,1,8-cineole,linalool oxide,1-octen-3-ol,(E)-3-hexenol]showed a decreasing trend during flowering stages. GC-IMS spectra showed that the samples at different flowering stages had their own characteristic peak regions,and also had common regions. The results of cluster analysis, PCA and OPLS-DA all showed that the samples at different flowering stages were distinguishable. OPLS-DA was used to screen 19 different components to distinguish different flowering stages,including γ-butyrolactone,1,8-cineole,ethyl hexanoate,etc. Conclusion: Rhododendri Mollis Flos samples at different flowering stages can be distinguished obviously,and the active substances in the volatile components are gradually dissipated with the degree of flower opening,which can provide reference for the improvement of material basis and the study of different flowering stages of Rhododendri Mollis Flos.
作者
冯靖雯
郭小红
张砚
杨昌林
刘友平
陈鸿平
FENG Jing-wen;GUO Xiao-hong;ZHANG Yan;YANG Chang-lin;LIU You-ping;CHEN Hong-ping(Key Laboratory of Standardization of Chinese Herbal Medicine,Ministry of Education,State Key Laboratory of Southwestern Chinese Medicine Resources,School of Pharmacy,Chengdu University of Traditional Chinese Medicine,Chengdu 611137,China;Sichuan Institute for Food and Drug Control,Chengdu 611731,China)
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2021年第10期85-92,共8页
Chinese Journal of Experimental Traditional Medical Formulae
基金
重庆市自然科学基金面上项目(cstc2019jcyj-msxmX0552)。
