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具有线粒体靶向功能的穿心莲内酯TPP-PEG-PE脂质体的制备及其作用于胃癌模型小鼠的机制研究 被引量:7

Preparation of andrographolide TPP-PEG-PE liposomes with mitochondrial targeting function and its mechanism in gastric cancer model mice
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摘要 目的制备穿心莲内酯(andrographolide,AP)(3-丙羧基)三苯基溴化膦[(3-carboxy propyl) triphenylphosph-onium bromide,TPP]-聚乙二醇(polyethyleneglycol,PEG)-聚乙烯(polyethylene,PE)脂质体(AP@TPP-PEG-PE#Lips),研究其体外释放、溶血性、线粒体靶向性、促胃癌细胞凋亡和作用胃癌模型小鼠的机制。方法采用薄膜水化法制备AP@TPP-PEG-PE#Lips,检测该脂质体的粒径、电势、显微电镜形态、稳定性、溶血性及体外释放情况;流式细胞仪测定胃癌细胞对脂质体的摄取情况,激光共聚焦显微镜研究其在线粒体的靶向性;评价AP@TPP-PEG-PE#Lips对胃癌细胞凋亡和胃癌模型小鼠的影响。结果 AP@TPP-PEG-PE#Lips粒径为(23.8±1.7)nm,Zeta电位为(30.2±1.1)m V,分布较为均匀,规则球形结构;体外试验表明,AP@TPP-PEG-PE#Lips溶血率低、缓释性能和血液相容性好;荧光试验结果显示,TPP-PEGPE#Lips可以促进药物的细胞摄取;AP@TPP-PEG-PE#Lips作用胃癌细胞和胃癌模型小鼠结果显示,AP@TPP-PEG-PE#Lips可以明显降低胃癌细胞线粒体膜电位,增加细胞内活性氧(reactiveoxygenspecies,ROS)含量,促进半胱氨酸蛋白酶-3(Caspase-3)的释放,增加促凋亡蛋白B淋巴细胞瘤-2(Bcl-2)和减少抗凋亡蛋白Bcl2-associatedX(Bax)的表达。结论 AP@TPP-PEG-PE#Lips可以有效的将药物递送到线粒体,增强药物促肿瘤细胞凋亡作用。 Objective To prepare andrographolide(AP) triphenyl phosphate(TPP)-polyethylene glycol(PEG)-polyethylene(PE) liposomes(AP@TPP-PEG-PE#Lips), and study its in vitro release, hemolysis, mitochondrial targeting, the mechanism of promoting gastric cancer cell apoptosis and acting on gastric cancer model mice. Methods The membrane hydration method was used to prepare AP@TPP-PEG-PE#Lips;The particle size, electric potential, micro-electron microscopic morphology, stability, hemolysis and in vitro release of the liposomes were detected;Flow cytometry was used to measure the uptake of gastric cancer cells to liposomes, and laser confocal microscopy to study its mitochondrial targeting;AP@TPP-PEG-PE#Lips in gastric cancer cells and effects of apoptosis and gastric cancer model mice were evaluated. Results The particle size of AP@TPP-PEG-PE#Lips is(23.8 ± 1.7) nm, the Zeta potential is(30.2 ± 1.1) mV, the distribution is relatively uniform, and the regular spherical structure;In vitro tests showed that AP@TPP-PEGPE#Lips had low hemolysis rate, sustained release performance and good blood compatibility;Fluorescence test results showed that TPP-PEG-PE#Lips can promote the cellular uptake of drugs;AP@TPP-PEG-PE#Lips act on gastric cancer cells and gastric cancer model mice, the results showed that AP@TPP-PEG-PE#Lips can significantly reduce the mitochondrial membrane potential of gastric cancer cells, increase intracellular reactive oxygen species(ROS) content, and promote cysteine The release of Caspase-3 increased the pro-apoptotic protein B lymphocyte tumor-2(Bcl-2) and decreased the expression of anti-apoptotic BCL2-Associated X(Bax) protein(P < 0.05). Conclusion AP@TPP-PEG-PE#Lips can effectively deliver the drug to the mitochondria and enhance the drug’s effect of promoting tumor cell apoptosis.
作者 安丽丽 孙贺军 孔永红 王新明 赵成龙 AN Li-li;SUN He-jun;KONG Yong-hong;WANG Xin-ming;ZHAO Cheng-long(Department of Pharmacy,Zhumadian Central Hospital,Zhumadian 463000,China;Department of Gastroenterology,Henan Provincial People’s Hospital,Zhengzhou 454002,China;Henan Academy of Chinese Medicine,Zhengzhou 450004,China)
出处 《中草药》 CAS CSCD 北大核心 2021年第7期1945-1956,共12页 Chinese Traditional and Herbal Drugs
基金 河南省卫生健康委基金项目(201702161)。
关键词 线粒体靶向 穿心莲内酯 TPP-PEG-PE 脂质体 细胞摄取 胃癌 体外释放 溶血性 细胞凋亡 薄膜水化法 活性氧 Caspase-3 Bcl-2 Bax mitochondrial targeting andrographolide TPP-PEG-PE liposome cell uptake gastric cancer in vitro release hemolytic apoptosis membrane hydration method reactive oxygen species Caspase-3 Bcl-2 Bax
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