摘要
【目的】细胞生长的每个阶段都离不开蛋白质相互作用,研究假眼小绿叶蝉侵染茶叶后差异基因参与的蛋白质相互作用对解析抗虫分子机理具有指导意义。【方法】对有无受假眼小绿叶蝉侵害的都匀毛尖茶树鸟王种进行转录组测序,基于同源比对算法构建0 h与12 h、0 h与24 h的差异基因蛋白质相互作用网络。【结果】0 h与12 h的差异基因共有212个互作,0 h与24 h的差异基因共有3551个互作,合并网络后得到3605个互作,并对网络进行拓扑属性分析,发现网络中蛋白质的度符合幂律分布。通过网络可以进行蛋白质的功能预测,并且对网络进行GO和KEGG分析发现差异基因的蛋白互作主要参与植物病原体互作,植物激素信号转导,DNA碱基切除修复、核酸切除修复、错配修复,亚麻酸和亚油酸代谢等生物过程,并且侵染24 h比侵染12 h需要启动更多的互作来抵御假眼小绿叶蝉的侵染。【结论】茶叶主要通过三个差异基因启动相应的互作来抵抗假眼小绿叶蝉的侵害,继续被侵害时将启动更多的互作,并通过产生相关次级代谢产物来抵御虫害。研究结果为探究鸟王种受假眼小绿叶蝉取食前后茶树抵御假眼小绿叶蝉侵染的代谢机理提供参考,为都匀毛尖病虫害防治、后续良种选育和引种奠定基础。
【Objective】The protein-protein interaction networks(PPINs)was applied to decipher the protective mechanism of tea plants in resisting the invasion by insects.【Method】RNA-seq was used to determine if a Camellia sinensis cv.Niaowang plant was infested by Empoasca vitis Gothe.PPINs of differential expression genes(DEGs)between 0 h and 12 h as well as 0 h and 24 h after the infestation were constructed based on Interolog.【Result】There were 212 PPIs between 0 h and 12 h,3551 PPIs between 0 h and 24 h,and 3605 PPIs in the merged network.The topological properties of the PPINs showed the degree of the protein distribution following the power-law model,and hence,their functions predictable.It was found that DEGs mainly partook in the plant-pathogen interactions,plant hormone signal transduction,DNA base excision repair,nucleotide excision repair,mismatch repair,linolenic acid and alpha-linoleic acid metabolism,and other biological processes through GO and KEGG analysis.Furthermore,the tea leaves that were infected for 24 h would require greater amounts of PPIs than those affected for 12 h to develop the defense mechanism to ward off the ill-effect brought by Empoasca vitis Gothe.【Conclusion】Tea plants mainly resist the invasion of Empoasca Vitis through the three differential genes to promote the corresponding interaction,and when tea plants continue to be invaded,more interactions will be initiated,or various secondary metabolites will be produced to resist pests.To resist the invasion of Empoasca Vitis,tea plant mainly promoted the PPIs of three differential genes.When invasion continuing,more interactions would be initiated,and various secondary metabolites would be produced to resist pests.
作者
王芬
裴会敏
文狄
陈志
李静
WANG Fen;PEI Huimin;WEN Di;CHEN Zhi;LI Jing(Department of Life Science and Agriculture,Qiannan Normal University for Nationalities,Duyun,Guizhou558000,China)
出处
《福建农业学报》
CAS
CSCD
北大核心
2021年第3期326-331,共6页
Fujian Journal of Agricultural Sciences
基金
贵州省科技厅基础研究计划项目(黔科合基础[2019]1298)
贵州省教育厅项目(黔教合KY字[2018]420、[2018]419和[2016]020)
国中医药办科技函([2018]132号、[2019]186号)
贵州省植物学重点支持学科开放基金(qnsyzw1809)
黔南民族师范学院科研创新团队项目(QNYSKYTD2018011、Qnsyk201605)
黔南民族师范学院项目(QNSY2018ZJ006、2019xjg0303、QNSY2018BS018)。
关键词
差异基因
蛋白质相互作用网络
假眼小绿叶蝉
鸟王种
differential expression genes
protein-protein interaction network
Empoasca vitis Gothe
Camellia sinensis cv.Niaowang
