摘要
目的探讨慢性丙型肝炎(CHC)患者干扰素调节因子(IRF)-3水平的变化及其与丙型肝炎病毒(HCV)RNA载量、肝脏损伤程度及干扰素抗病毒疗效的关系。方法检测57例CHC患者抗病毒治疗前及26名体检健康者(正常对照组)血浆IRF-3、β-干扰素(IFN-β)水平,同时检测丙氨酸氨基转移酶(ALT)活性、Ⅳ型胶原(CⅣ)水平及HCV RNA载量。根据ALT、CⅣ和HCV RNA载量分别分组;根据抗病毒疗效分为应答组和无应答组。采用Spearman秩相关分析评估各项目之间的相关性。结果CHC组血浆IRF-3、IFN-β水平明显低于正常对照组(P<0.01)。ALT升高组与ALT正常组之间、CⅣ升高组与CⅣ正常组之间、HCV RNA高载量组与HCV RNA低载量组之间血浆IRF-3水平差异均有统计学意义(P<0.05)。Spearman秩相关分析结果显示,CHC患者IRF-3与IFN-β呈正相关(r=0.930,P<0.01),与HCV RNA载量、ALT、CⅣ均呈负相关(r值分别为-0.321、-0.290、-0.345,P<0.05)。应答组血浆IRF-3水平低于无应答组(P<0.05),血浆IFN-β水平2个组之间差异无统计学意义(P>0.05)。结论CHC患者血浆IRF-3水平与HCV RNA载量、肝细胞损伤程度及纤维化程度有关,对干扰素疗效的判断也有一定的价值。
Objective To investigate the changes of interferon regulatory factor(IRF)-3 level in patients with chronic hepatitis C(CHC)and its relationship with hepatitis C virus(HCV)RNA load,the degree of liver injury and antiviral efficacy of interferon.Methods The levels of IRF-3 and beta-interferon(IFN-β)were determined for 26 healthy subjects and 57 patients with CHC.The alanine aminotransferase(ALT)activity,typeⅣcollagen(CⅣ)level and HCV RNA load in plasma of CHC patients were also determined.According to the ALT activity,CⅣlevel,HCV RNA load and the efficacy of interferon therapy,the patients were classified into 2 groups.Spearman rank correlation analysis was used to evaluate the correlation among these parameters.Results The levels of plasma IRF-3 and IFN-βin CHC group were lower than those in healthy control group(P<0.01).The levels of plasma IRF-3 had statistical significance between high ALT activity group and normal ALT activity group,high CⅣlevel group and normal CⅣlevel group,high HCV RNA load group and low HCV RNA load group(P<0.05).Spearman rank correlation analysis showed that the IRF-3 level was positively correlated with IFN-βin CHC patients(r=0.930,P<0.01),and showed negative correlations with HCV RNA load,ALT activity and CⅣlevel(r values were-0.321,-0.290 and-0.345,respectively,P<0.05).The IRF-3 level in response group was lower than those in non-response group(P<0.05),and there was no statistical significance in IFN-βlevels between the 2 groups(P>0.05).Conclusions The level of plasma IRF-3 in CHC patients is related to HCV RNA load,the degree of hepatic cell damage and the degree of hepatic fibrosis,and it is valuable to judge the efficacy of IFN.
作者
叶松道
陈允国
叶宣梅
YE Songdao;CHEN Yunguo;YE Xuanmei(Center of Medical Laboratory,the Second Affiliated Hospital of Wenzhou Medical University,Wenzhou 325027,Zhejiang,China)
出处
《检验医学》
CAS
2021年第5期486-489,共4页
Laboratory Medicine
基金
浙江省医药卫生科技计划项目(2016KYB191)
温州市科技计划项目(Y20160501)。
