摘要
【目的】鉴定分离自新疆某辐射污染区的丝状真菌F161,研究其吸附锶特性。【方法】采用形态学观察、分子生物学鉴定及碳源利用实验鉴定菌种;采用压力试验和吸附试验研究特性,对锶的吸附测定采用ICP-MS,对放射性90锶的吸附的测定采用液闪检测仪。【结果】菌株F161鉴定为青霉属真菌;菌株F161能够耐受10 KGy剂量的钴源照射,对重金属离子Cr3+、Zn2+、Co2+、Pb2+、Hg2+均有较高耐受性。在pH 7.0,28℃下,伴随生长3~4 d,F161对Sr2+吸附效率最高能达到36.67%,对放射性90锶的吸附率为49.45%。【结论】F161是1株对放射性核素污染具有修复能力的土著丝状真菌,在有较高的重金属离子混合污染的放射性环境中具有一定的修复应用潜力。
【Objective】To identify the filamentous fungus F161 isolated from a radiation-contaminated area in Xinjiang and study its characteristics of strontium adsorption.【Methods】Morphological observation,molecular biology and carbon source utilization were used to identify F161 and the characteristics were studied by means of pressure test and adsorption experiment,and the adsorption of strontium was determined by ICP-MS,and the adsorption of radioactive strontium 90 was determined by liquid flash detector.【Results】The strain F161 was identified as Penicillium sp.by morphological,molecular biological identification and carbon source utilization experiments.The F161 strain could tolerate 10 KGy dose irradiation of radioactive cobalt,and also had high tolerance of heavy mental ions Cr3+,Zn2+,Co2+,Pb2+,Hg 2+.At pH 7.0 and 28℃for 3-4 days with growth,the adsorption efficiency of Sr2+by F161 was up to 36.67%,and the adsorption efficiency of radioactive strontium 90 was 49.45%.【Conclusion】F161 is an indigenous filamentous fungus with the ability to treat radionuclide contamination,which has the potential of remediation application in radioactive environment with high contamination of heavy metal ions.
作者
张丽娟
唐琦勇
谢玉清
顾美英
王博
朱静
宋素琴
黄伟
张志东
王玮
ZHANG Lijuan;TANG Qiyong;XIE Xuqing;GU Meiying;WANG Bo;ZHU Jing;SONG Suqin;HUANG Wei;ZHANG Zhidong;WANG Wei(Xinjiang Laboratory of Special Environmental Microbiology/ Institute of Applied Microbiology, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China)
出处
《新疆农业科学》
CAS
CSCD
北大核心
2021年第5期882-892,共11页
Xinjiang Agricultural Sciences
基金
国家自然科学基金(31560023)
国家高技术研究发展计划(2012AA063503)。
关键词
耐辐射
青霉
放射性核素
锶
生物吸附
radiation-resistant
Penicillium sp.
radionuclide
strontium
biosorptio
