摘要
以新疆某羊场疑似传染性胸膜肺炎和羊口疮混合感染的绵羊作为研究对象,对其感染情况和发病症状进行调查记录;对死亡的羊只进行解剖;无菌采集病羊肺脏组织后,通过细菌分离鉴定和绵羊支原体PCR以及羊口疮病毒PCR的方法进行实验室病原诊断。结果表明:死亡羊只嘴角结痂、口腔糜烂,胸膜与心包膜、胸膜发生粘连,肺实质发生肝变,切面呈大理石样,具有典型的羊口疮和胸膜肺炎的特征;绵羊支原体、羊口疮病毒和曼氏杆菌PCR检测结果均为阳性,小反刍兽疫和口蹄疫核酸检测结果为阴性。该病例最终诊断为绵羊肺炎支原体、羊口疮病毒和溶血性曼氏杆菌的混合感染。本研究通过对绵羊支原体肺炎、羊口疮和溶血性曼氏杆菌的临床症状、病理变化、实验室诊断等方面的介绍,可为今后该病的防控工作提供指导和借鉴。
The sheep suspected of mixed infection of contagious pleuropneumonia and ovine aphtha in a sheep farm in Xinjiang were taken as the research object,and the infection and symptoms were investigated and recorded;the dead sheep were dissected;the lung tissues of the diseased sheep were collected aseptically,and the pathogen was diagnosed by bacteria isolation and identification,mycoplasma spontaneum PCR and ovine aphtha virus PCR.The results showed that the dead sheep had scab on the corners of mouth,oral erosion,adhesion between pleura and pericardium,pleura,liver change in lung parenchyma,marbling in section,having typical characteristics of contagious pleuropneumonia and ovine aphtha;the PCR results of mycoplasma ovipne-umoniae,ovine aphtha virus and mansoni were all positive,but the nucleic acid detection results of peste des petits ruminants and foot-and-mouth disease were negative.The final diagnosis indicated that the case was the mixed infection of mycoplasma ovipneumoniae,ovine aphtha virus and Mannheimia haemolytica.Through the introduction of clinical symptoms,pathological changes and laboratory diagnosis of contagious pleuropneumonia,ovine aphtha and Mannheimia haemolytica,this study could provide guidance and reference for the prevention and control of the disease in the future.
作者
金映红
汪阳
沙吾尔江·阿不都力艾拉
古丽·巴哈吾拉音
沈辰峰
郭会玲
薛晶
赵洁雅
梁纪元
王杰
夏俊
JIN Yinghong;WANG Yang;Shaurjiang Abduly Ella;Gully Bahawula;SHEN Chenfeng;GUO Huiling;XUE Jing;ZHAO Jieya;LIANG Jiyuan;WANG Jie;XIA Jun(Institute of Veterinary Medicine,Xinjiang Academy of Livestock Sciences/Research Center for Animal Clinical Medicine,Xinjiang Academy of Livestock Sciences,Urumqi Xinjiang 830000;Xinjiang Agricultural University,Urumqi Xinjiang 830000;Xinjiang Animal Health Supervision Institute,Urumqi Xinjiang 830000;Animal Husbandry and Veterinary Station in Beidaqu Township of Yanqi County,Yanqi Xinjiang 841100)
出处
《现代农业科技》
2021年第10期170-173,共4页
Modern Agricultural Science and Technology
基金
天山创新团队计划项目“动物病毒病防控技术研究与应用创新团队”(2020D14003)
天山青年计划“优秀青年科技人才培养项目”(2018Q045)
农区高效肉羊品种选育推广体系(xjnqry-g-2006)。
关键词
绵羊肺炎支原体
羊口疮
溶血性曼氏杆菌
混合感染
诊断
防治
mycoplasma ovipneumoniae
ovine aphtha
Mannheimia haemolytica
mixed infection
diagnosis
prevention and control
