摘要
以红醋栗不同组织部位为外植体,采用不同的消毒药剂、消毒方式及消毒时间对其进行试验研究,结果表明:红醋栗离体初代培养以半木质化茎段为外植体,消毒药剂为0.1%升汞(HgCL2)+2滴吐温溶液,通过手摇容器晃动的方式消毒8 min的试验效果最佳。
The seletion of red currant explants in primary tissue culture and the disinfection effects of different disinfectants,disinfecting methods and disinfecting time on the explants were studied in this paper.The results showed that:Semi-lignified stem segments were the proper explants for primary tissue culture of red currant,and disinfecting the explants with 0.1%mercuric chloride and 2 drops of twain solution by manual shaking the containers for 8 minutes could achieve the best disinfection effect.
作者
朱虹
ZHU Hong(Institute of Dryland Agriculture and Forestry of Liaoning Province,Liaoning Chaoyang 122000)
出处
《林业科技》
2021年第3期15-17,共3页
Forestry Science & Technology
关键词
红醋栗
外植体消毒
消毒方法
诱导率
Red currant
Explant disinfection
Disinfection methods
Induction rate
