摘要
为探究副猪嗜血杆菌(Haemophilus parasuis)sodA基因编码的超氧化物歧化酶(Superoxide Dismutase,SOD)的活性及理化性质,设计特异性引物从HPS中扩增得到目的基因sodA,利用原核表达系统对HPS SOD蛋白进行表达,通过硫酸铵分级沉淀、Sephacryl S-300凝胶过滤层析以及DEAE Sepharose Fast Flow阴离子交换层析等方法对表达蛋白进行了分离纯化,利用邻苯三酚自氧化法对纯化产物进行SOD酶活性检测,通过HPSEC法、火焰原子吸收分光光度法对其理化性质进行了初步探究。结果表明,表达的重组HPS SOD蛋白质为可溶性蛋白质,单亚基分子质量为26 ku,纯化后目的蛋白质纯度为95%,纯化后的重组HPS SOD蛋白质比活性为215.9 U/mg,经HPSEC法检测分析,重组HPS SOD呈多聚体,通过火焰原子吸收分光光度法检测纯化的重组HPS SOD中锰元素含量为0.71 mg/L。
In order to study the activity and physiological function of the SOD protein encoded by the sodA gene of Haemophilus parasu⁃is,the sodA gene was amplified from HPS by the specific primers and expressed in Escherichia coli prokaryotic expression system.The expressed protein was purified by the ammonium sulfate fractionation,Sephacryl S-300 gel filtration chromatography and DEAE Sep⁃harose Fast Flow anion exchange chromatography.The activity of the purified protein was determined by the pyrogallol autoxidation method and the physicochemical properties were studied by HPSEC and AAS.The results showed that the recombinant HPS SOD pro⁃tein was a soluble protein with a size of 26 ku.The purity of the target protein was 95%after purification,and its activity was measured to be 215.9 U/mg by pyrogallol autoxidation assay.HPSEC analysis indicated that the recombinant HPS SOD was a multimer,and the manganese content in the purified HPS SOD was detected to be 0.71 mg/L by Flame Atomic Absorption Spectrophotometry.
作者
胡基雄
王席
李国攀
荣俊
HU Ji-xiong;WANG Xi;LI Guo-pan;RONG Jun(College of Life Science,Yangtze University,Jingzhou 434025,Hubei,China;Jingzhou Changxin Biotechnology Co.,Ltd.,Jingzhou 434025,Hubei,China)
出处
《湖北农业科学》
2021年第10期142-147,共6页
Hubei Agricultural Sciences
基金
高校教育综合奖补项目(2018-702090250401)。
