摘要
目的探讨不同疾病状态下SLE患者外周血髓样树突状细胞(mDC)和浆样树突状细胞(pDC)的频率及肾脏损伤的关系。方法应用流式细胞术检测102例SLE患者和10名健康对照组外周mDC和pDC的频率,分析与疾病活动度及肾脏损伤的关系。定量资料采用中位数(第25百分位数,第75百分位数)[M(P25,P75)]表示,非正态分布的2组计量资料采用Mann-Whitney U检验,2组计量资料的相关性采用Spearman秩相关分析,相关因素分析采用多元线性回归进行统计分析。结果102例SLE患者外周血pDC[14.00%(7.92%,19.65%)和24.55%(19.68%,32.90%),Z=-3.163,P<0.01]和mDC[21.25%(13.28%,32.83%)和34.85%(24.58%,41.93%),Z=-2.607,P<0.01]频率显著低于健康对照组,12例初发SLE患者pDC[9.09%(7.31%,17.38%)和24.55%(19.68%,32.90%),Z=-3.033,P<0.01]和mDC[9.40%(7.88%,21.60%)和34.85%(24.58%,41.93%),Z=-3.231,P<0.01]频率也显著低于健康对照组。校正混杂因素后,多因素分析示SLEDAI水平是影响pDC(P=0.019)和mDC(P<0.01)频率的主要因素。活动期LN患者pDC频率[8.02%(1.00%,10.40%)和14.60%(1.73%,38.0%),P=0.045]及mDC[8.80%(5.20%,13.20%)和13.90%(5.27%,40.20%),P=0.038]频率均显著低于非LN活动期患者。此外,活动期LN患者pDC[8.02%(2.25%,9.97%)和16.70%(11.80%,24.60%),Z=-2.490,P=0.015]和mDC[8.80%(5.99%,12.80%)和20.20%(11.20%,42.80%),Z=-2.226,P=0.029]频率也均显著低于稳定期LN患者。mDC频率与补体C3(r=0.455,P<0.01)和C4(r=0.289,P<0.01)水平呈正相关。结论SLE患者mDC和pDC频率存在明显异常,并与疾病活动密切相关。另外,pDC和mDC可能参与LN的发生发展。
Objective To investigate the frequency of myeloid dendritic cells(mDC)and plasmacytoid dendritic cells(pDC)in peripheral blood of patients with systemic lupus erythematosus(SLE)and their relationship with renal injury.Methods The frequency of peripheral mDC and pDC in 102 SLE patients and 10 healthy controls were detected by flow cytometry.The quantitative data were expressed by[M(P25,P75)].The measurement data of the two groups with non-normal distribution was analyzed by Mann Whitney U test.The correlation between the two groups was analyzed by Spearman rank correlation analysis and multiple linear regression.Results The frequency of pDC[14.00%(7.92%,19.65%)vs 24.55%(19.68%,32.90%),Z=-3.163,P<0.01]and mDC[21.25%(13.28%,32.83%)vs 34.85%(24.58%,41.93%),Z=-2.607,P<0.01]in the peripheral blood of 102 patients with SLE were significantly lower than those of healthy controls.The frequency of pDC[9.09%(7.31%,17.38%)vs 24.55%(19.68%,32.90%),Z=-3.033,P=<0.01]and mDC[9.40%(7.88%,21.60%)vs 34.85%(24.58%,41.93%),Z=-3.231,P<0.01]in 12 patients with newly diagnosed SLE were also significantly lower than those in healthy controls.After adjustedfor confounding factors,multivariate analysis showed that SLEDAI level was the main factor influencing the frequency of pDC(P=0.019)and mDC(P<0.01).In addition,pDC[8.02%(2.25%,9.97%)vs 16.70%(11.80%,24.60%),Z=-2.490,P=0.015]and mDC[8.80%(5.99%,12.80%)vs 20.20%(11.20%,42.80%),Z=-2.226,P=0.029]in patients with active LN were also significantly lower than that of patients with stable LN.The mDC frequency was positively correlated with the levels of complement C3(r=0.455,P<0.01)and C4(r=0.289,P,P<0.01).Conclusion The frequency of mDC and pDC in SLE patients is significantly abnormal,which is closely related to disease activity.In addition,pDC and mDC may be involved in the occurrence and development of LN.
作者
俱博苗
郑健
王静
吕晓虹
胡楠
张竞
朱丽
蒲丹
罗静
王妍华
郝志明
何岚
Ju Bomiao;Zheng Jian;Wang Jing;Lyu Xiaohong;Hu Nan;Zhang Jing;Zhu Li;Pu Dan;Luo Jing;Wang Yanhua;Hao Zhiming;He Lan(Department of Rheumatology and Immunology,the First Affiliated Hospital of Xi'an Jiaotong University,Shaanxi,710061)
出处
《中华风湿病学杂志》
CAS
CSCD
北大核心
2021年第4期225-230,共6页
Chinese Journal of Rheumatology
基金
西安交通大学第一附属医院院级课题(面上项目2018MS-16)。
关键词
红斑狼疮
系统性
狼疮肾炎
树突细胞
Lupus erythematosus,systemic
Lupus nephritis
Dendritic cells
