摘要
采用超高效液相色谱荧光检测法(UPLC-FLD)检测不同品种初榨橄榄油中6种多酚化合物的含量,并测定样品的总多酚含量及抗氧化活性,同时对多酚组分与抗氧化能力的相关性进行分析。结果表明:色谱条件为采用Cadenza色谱柱(150 mm×4.6 mm,5μm),柱温35℃,以0.1%甲酸-水(A)-乙腈(B)为流动相进行梯度洗脱,荧光光谱激发波长为278 nm,发射波长为339 nm。在此条件下,初榨橄榄油样品中6种多酚化合物可在17 min内得到较好分离,并在较宽的浓度范围内呈良好的线性关系(R2≥0.9995),且精密度高,日内精密度相对标准偏差(RSD)≤4.91%,日间精密度RSD≤5.41%,加标回收结果准确可靠,平均回收率93.42%~119.86%,RSD≤6.26%。不同初榨橄榄油样品中羟基酪醇、酪醇、香草酸、松脂酚及总多酚含量差异较大,而橄榄苦苷和女贞苷含量最低,且样品间差异较小。初榨橄榄油多酚提取物具有良好的抗氧化活性,但不同品种间具有较大差异,ABTS法测定的总抗氧化能力为0.95~2.71 mmol·L^(-1) Trolox,DPPH自由基清除率为7.22%~66.23%,抑制羟自由基能力为13.24~136.06 U·mL^(-1),其中Coratina的抗氧化活性最强,总多酚含量与提取物总抗氧化能力呈显著正相关。
An ultra high performance liquid chromatography(UPLC)coupled with fluorescence detector(FLD)was used to determine the contents of 6 selected phenolic compounds in different samples of virgin olive oil.The total polyphenols content and antioxidant activity of these samples were also detected.The chromatographic column used was Cadenza(150 mm×4.6 mm,5μm)and the mobile phase was composed of 0.1%formate-water(A)and acetonitrile(B).Column temperature was at 35℃.The excitation wavelength and emission wavelength were 278 nm and 339 nm.Under the conditions,6 phenolic compounds in virgin olive oil could be separated well within 17 min,with a wider range of concentration,good linear relationship(R2≥0.9995),high precision with the relative standard deviation(RSD)of intra-day precision≤4.91%,RSD of inter-day precision≤5.41%,and reliable recovery with the average recovery rate of 93.42%-119.86%,RSD≤6.26%.The contents of hydroxytyrosol,tyrosol,vanillic acid,pinoresinol and total polyphenols in different virgin olive oil samples were significantly different,while the contents of oleuropein and ligstroside were the lowest and the differences between samples were relatively small.Polyphenols extracts of virgin olive oil have good antioxidant activity,but significant differences between different samples were detected.The total antioxidant capacities of the samples were from 0.95 to 2.71 mmol·L^(-1) Trolox determined by ABTS method.DPPH free radical scavenging activities of samples were 7.22%-66.23%.Hydroxyl free radical clearance capabilities of samples were 13.24-136.06 U·mL^(-1).Coratina showed the strongest antioxidant activity among samples.The content of total polyphenols was significantly correlated to total antioxidant capacity of the extracts of virgin olive oil.
作者
李雪
张玉
王君虹
朱作艺
孙素玲
刘志
朱申龙
沈国新
Agusti ROMERO
王伟
LI Xue;ZHANG Yu;WANG Junhong;ZHU Zuoyi;SUN Suling;LIU Zhi;ZHU Shenlong;SHEN Guoxin;Agusti ROMERO;WANG Wei(Institue of Agro-Product Safety and Nutrition, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China;Key Laboratory of Information Traceability for Agricultural Products, Ministry of Agriculture and Rural Affairs of China, Hangzhou 310021, China;International Joint Laboratory for Quality and Nutrition of Woody Oils, Hangzhou 310021, China;College of Agriculture and Biotechnology, Hunan University of Humanities, Science and Technology, Loudi 417000, China;Institute of Agriculture and Food Research and Technology, Catalonia 08140, Spain)
出处
《浙江农业学报》
CSCD
北大核心
2021年第5期907-915,共9页
Acta Agriculturae Zhejiangensis
基金
浙江省农业科学院青年人才培养项目(2020)
浙江省农业科学院B类学科建设项目
浙江省农业科学院国际合作项目(2019)。
