摘要
目的分析广东省梅州市五华县暴发的一起人间布鲁氏菌病的流行及溯源情况,为布病防治提供科学依据。方法采用描述性流行病学方法对广东省梅州市五华县发生的布鲁氏菌病疫情进行分析;应用分子生物学方法对血液及羊奶样品中的布鲁氏菌进行检测。根据AMOS-PCR和血清凝集试验等方法对分离菌株进行种型鉴定,并采用MLST技术对分离菌株进行种间遗传关系分析。结果在血液及羊奶样品中均检测到布鲁氏菌Omp22和16S rRNA基因。全血样品中共分离到14株布鲁氏菌,且均为羊种3型布鲁氏菌。经MLST技术聚类分析后,14株羊种布氏菌分离株与11株各生物种型标准株间的相似值介于82.0%~100.0%。结论广东省梅州市五华县暴发的人间布鲁氏菌病疫情,其引起该病的主要原因是生饮鲜羊奶所致,这为广东地区布鲁氏菌病的防控提供了有利的数据。
The objective of this study was to analyze an outbreak of human brucellosis in Wuhua County,Meizhou City,Guangdong Province,and to provide a scientific basis for the prevention and control of brucellosis.Descriptive epidemiological methods were used to analyze the brucellosis epidemic in Wuhua County.Molecular biological methods were used to detect Brucella in blood and fresh goat milk samples.Type identification of isolated strains was performed on the basis of AMOS-PCR and serum agglutination testing.MLST technology was used to analyze the genetic relationships among the isolated strains.The Brucella Omp22 and 16S rRNA genes were detected in the blood and goat milk samples.A total of 14 strains of Brucella were isolated from the whole blood samples,all of which were Brucella melitensis type 3.The similarity among the 14 isolated strains and 11 standard strains ranged from 82.0%to 100.0%.The outbreak of human brucellosis was mainly caused by drinking fresh goat milk in Wuhua County.This study provides data that may aid in the prevention and control of brucellosis in Guangzhou.
作者
孙长云
黄天鹏
韦入丰
张薇
邓爱萍
彭晓放
曾灵风
张少忠
黄元祥
武婕
SUN Chang-yun;HUANG Tian-peng;WEI Ru-feng;ZHANG Wei;DENG Ai-ping;PENG Xiao-fang;ZENG Ling-feng;ZHANG Shao-zhong;HUANG Yuan-xiang;WU Jie(Guangdong Center for Disease Control and Prevention,Guangzhou 511430,China;College of Veterinary Medicine,Inner Mongolia Agricultural University,Hohhot 010018,China;South District of Sun Yat Sen Memorial Hospital,Guangzhou 511430,China;Wuhua Center for Disease Control and Prevention,Meizhou 514411,China)
出处
《中国人兽共患病学报》
CAS
CSCD
北大核心
2021年第5期467-472,共6页
Chinese Journal of Zoonoses
基金
广东省自然科学基金项目(No.2019A1515012121)。
