摘要
目的研究山萸肉饮片中马钱苷的浓缩工艺。方法以马钱苷为指标,采用超滤-纳滤联用技术,在明确跨膜压力差、pH值因素影响的基础上,采用Box-Behnken中心组和设计建立数学模型,考察膜孔径、跨膜压力差、pH值,优选山萸肉提取液的浓缩工艺。结果超滤可去除多糖,改善溶液可滤性;马钱苷纳滤浓缩工艺为膜孔径400 Da,pH值6.7,跨膜压力差1.20 MPa,马钱苷平均截留率为(91.9±1.7)%,与理论截留率93.6%相近。结论超滤-纳滤联用精制山萸肉提取液中马钱苷,工艺稳定可行,可避免热处理引起的成分转化损失。
Objective To explore the Optimize concentrate process of loganin from Cornus officinalis by ultrafiltration-nanofiltration coupling technology.Methods Based on single factor test,nanofiltration membrane pore size,transmembrane pressure difference and pH were selected as independent variables,and the rejection of loganin was used as dependent variables.Response surface methodology of Box-Behnken Design was applied to optimize the concentrate process of loganin from Cornus officinalis.Results Ultrafiltration can remove polysaccharides and improve the filtrability of the solution.The optimum nanofiltration concentrate conditions were nanofiltration membrane pore size 400 Da,pH 6.7 and the transmembrane pressure difference was 1.20 MPa.The average cut-off rate of loganin was(91.9±1.7)%,which was close to the theoretical cut-off rate of 93.6%.Conclusion The loganin from Cornus officinalis has been concentrated efficiently by the combination of ultrafiltration and nanofiltration.The coupling technology is stable and feasible,whinc could avoid the transformation loss of heat-sensitive ingredients.
作者
陈骁鹏
吴芸
叶慧
Chen Xiaopeng;Wu Yun;Ye Hui(Taizhou Institute for Food and Drug Control,Taizhou 225300,China)
出处
《国际中医中药杂志》
2021年第4期363-368,共6页
International Journal of Traditional Chinese Medicine
基金
泰州市市场监督管理局质量发展与能力提升项目(202008)。
关键词
山茱萸
马钱苷
纳滤
超滤
中药工艺改进
Corni fructus
Loganin
Nanofiltration
Ultrafiltration
Technology improving(TCD)
