摘要
肺癌是世界上死亡率最高的恶性肿瘤,肿瘤内部存在着明显的异质性,目前研究认为这种异质性在肺癌研究中有重要的临床意义。对于这种异质性的研究通常使用测序技术,而一般测序技术只能从整体的角度解释样本之间的差异,其分辨率不足以描绘单个细胞之间的差异。因此人们迫切希望从单细胞层面来获得肿瘤微环境中的细胞类型、状态和亚群分布以及细胞之间的通讯行为等信息。单细胞测序技术解决了这一难题。利用该技术将有助于深入了解肺癌的发生和发展机制,并发现新的诊断标记物和治疗靶点,为今后肺癌患者的精准治疗提供理论参考。本文介绍了单细胞测序技术的发展,并重点讨论了其在肺癌肿瘤异质性、肺癌肿瘤微环境、肺癌侵袭和转移以及治疗反应和耐药等方面的研究。
Lung cancer is the malignant tumor with the highest mortality rate in the world.Heterogeneity of lung cancer,usually studied by sequencing technology,is considered to have important clinical significance in current studies.However,general sequencing technology can only explain the differences between samples integrally and its resolution is not enough to describe the differences between the individual cells.Therefore,people urgently hope to understand the cell type,state,subgroup distribution in the tumor microenvironment and the communication behavior between cells in the single cell level.Single-cell sequencing technology solves this problem.Using this technique will contribute to further understanding the mechanism of the occurrence and development of lung cancer,discovering new diagnostic markers and therapeutic targets,and providing theoretical references for the precise treatment of lung cancer patients in the future.This article reviews the progress of single-cell sequencing technology and focuses on its research on lung cancer tumor heterogeneity,tumor microenvironment,invasion and metastasis,treatment response,and drug resistance.
作者
俞孟军
谭金晶
王敬慧
Mengjun YU;Jinjing TAN;Jinghui WANG(Department of Medical Oncology,Beijing Tuberculosis and Thoracic Tumor Research Institute,Beijing Chest Hospital,Capital Medical University,Beijing 101149,China;Department of Cellular and Molecular Biology,Beijing Tuberculosis and Thoracic Tumor Research Institute,Beijing Chest Hospital,Capital Medical University,Beijing 101149,China;Cancer Research Center,Beijing Tuberculosis and Thoracic Tumor Research Institute,Beijing Chest Hospital,Capital Medical University,Beijing 101149,China)
出处
《中国肺癌杂志》
CAS
CSCD
北大核心
2021年第4期279-283,共5页
Chinese Journal of Lung Cancer
关键词
单细胞测序
肺肿瘤
肿瘤异质性
肿瘤微环境
精准治疗
Single-cell sequencing
Lung neoplasms
Tumor heterogeneity
Tumor microenvironment
Precision treatment
