摘要
本研究利用花粉管通道法,将高粱无融合生殖系SSA-1(Shanxi sorghum apomict-1)的全基因组DNA导入水稻‘辽盐28号’、‘辽盐6号’两个品种,子代的遗传性状发生了明显变化,经过连续自交选择和鉴定,获得了27个表型稳定的D4代株系。通过田间种植,对导入株系的抽穗期、分蘖数、株高、千粒重等农艺性状的表型进行了统计分析。结果表明:D4代株系的农艺性状存在广泛变异,部分D4代株系茎秆、穗粒颜色更偏向供体高粱,表明高粱全基因组DNA成功导入受体细胞,并对受体基因的表达产生了影响,与受体亲本相比,导入株系在抽穗期、分蘖数、株高、每穗粒数、每穗粒重、穗长、千粒重等主要性状上都表现出明显变异。进一步对变异株系分析筛选有助于挖掘新种质材料,为选育水稻新品种提供丰富的种质资源。
In this study,the whole genome DNA of sorghum apomixes line SSA-1(Shanxi sorghum apomict-1)was injected into two rice varieties of‘Liaoyan28’and‘Liaoyan6’using the pollen tube pathway method.The genetic characters of the progeny changed obviously.By self-selection and identification,27 phenotypic stable D4 generation lines were obtained.Through field planting,the phenotypes of agronomic traits such as heading date,tiller number,plant height,and 1000-grain weight of the introduced lines were statistically analyzed.The results showed that the agronomic traits of the D4 generation lines were widely varied,some D4 generation lines were more inclined to donor sorghum in culm and spike color,which indicated that the whole genome DNA of sorghum was successfully introduced into the receptor cells,and the expression of the receptor gene was affected.Compared with the recipient parents,the introduced lines showed significant variations in the main traits such as heading date,tiller number,plant height,grain number per ear,grain weight per ear,ear length,and thousand grain weights.Further analysis and selection of mutant lines is helpful to discover new germplasm materials and provide abundant germplasm resources for breeding new rice varieties.
作者
李广信
王广元
于晓慧
梅青
王国鹏
Li Guangxin;Wang Guangyuan;Yu Xiaohui;Mei Qing;Wang Guopeng(College of Agriculture,Shanxi Agricultural University,Taiyuan,030031)
出处
《分子植物育种》
CAS
北大核心
2021年第9期3046-3052,共7页
Molecular Plant Breeding
基金
山西省重点研发计划项目(201603D221003-3
201903D221093)
山西省农业科学院生物育种工程项目(17yzgc087)
山西省农业科学院作物科学研究所青年基金项目(ZQ1803)共同资助。
关键词
高粱无融合生殖系
花粉管通道法
主要性状
遗传变异
Sorghum apomixes line
Pollen tube pathway method
Main characters
Genetic variation
