摘要
磷脂酶C(phospholipase C,PLC)广泛参与植物的生命活动和代谢过程,在抵御真菌感染的过程中发挥重要作用。本研究基于非洲菊转录组测序数据,鉴定出13个非洲菊PLC基因家族成员,其中NPC有3个,PI-PLC有10个。非洲菊PLC的蛋白序列长度在98~594 aa之间,蛋白理论分子量为11410.25~67998.85 kDa,等电点为4.74~9.59,均为不稳定亲水蛋白。亚细胞定位预测结果显示,13个成员分别定位于细胞壁、细胞膜、细胞质、叶绿体和细胞核中,表明不同成员功能上可能存在多样性。进化树分析表明,13个非洲菊PLC蛋白可分为2个亚组,进化关系较近的蛋白结构组成相似。非洲菊PLC家族成员在拟南芥中的同源蛋白PLC2、PLC4、AT2G40116可相互作用。非洲菊转录组的FPKM值分析表明,GjPLC2、GjPLC3、GjPLC8、GjPLC10在非洲菊中表达量高,感病植株中变化大,可能在抵御真菌感染过程中发挥重要作用。研究结果可为进一步研究非洲菊PLC基因的生物学功能提供依据。
Phospholipase C(PLC)is widely involved in the life activities and metabolism processes of plants,and plays an important role in the process of resisting fungal infection.Based on the results of the transcriptome sequencing in Gerbera jamesonii,13 members of G.jamesonii PLC gene family,including 3 NPC and 10 PI-PLC were identified in the study.The sequences of G.jamesonii PLC proteins ranged from 98 to 594 aa in length,theoretical molecular weight varied from 11410.25 to 67998.85 kDa,isoelectric point in the range of 4.74 to 9.59,and all were unstable hydrophilic proteins.The prediction results of subcellular localization showed that the 13 members were located in the cell wall,cell membrane,cytoplasm,chloroplast and nucleus,indicating that there might be diversity in functions of different members.Evolutionary trees analysis showed that 13 PLC proteins of G.jamesonii were divided into two subgroups,the structure and composition of proteins with relatively close evolutionary relationship were similar.G.jamesonii’s homologous protein PLC2,PLC4 and AT2G40116 in Arabidopsis thaliana can interact with each other.The FPKM value analysis of G.jamesonii transcription showed that GjPLC2,GjPLC3,GjPLC8 and GjPLC10 were highly expressed in G.jamesonii and changed greatly in infected plants,which may play an important role in preventing fungal infection.These results could provide the basis for further study of the biological function of G.jamesonii PLC genes.
作者
王秀美
倪珊珊
李乾玉
李小芳
林争春
陈裕坤
林玉玲
赖钟雄
杜迎刚
WANG Xiumei;NI Shanshan;LI Qianyu;LI Xiaofang;LIN Zhengchun;CHEN Yukun;LIN Yuling;LAI Zhongxiong;DU Yinggang(Institute of Horticultural Biotechnology,College of Horticulture,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China;Facility Horticulture Laboratory of Universities in Shandong,Weifang University of Science and Technology,Shouguang,Shandong 262700,China)
出处
《热带作物学报》
CSCD
北大核心
2021年第5期1267-1273,共7页
Chinese Journal of Tropical Crops
基金
福建省重大农业科技专项(No.2015NZ0002-1)
福建农林大学科技创新基金(No.CXZX2017189)
潍坊科技学院三大平台科研项目(No.2018YY010)。
关键词
非洲菊
磷脂酶C
基因家族
进化分析
Gerbera jamesonii Bolus
Phospholipase C
gene family
evolutionary analysis
