摘要
目的分析北京市海淀区新型冠状病毒肺炎(COVID-19)患者及其家庭成员中常见呼吸道病原体的流行情况,比较混合感染患者与只感染新型冠状病毒(SARS-CoV-2)患者的疾病严重程度差异。方法使用多重荧光定量PCR方法对2020年1—6月北京市海淀区疾病预防控制中心检测的所有COVID-19患者及其家庭成员同期采集的样本进行呼吸道病原谱检测,并比较混合感染与非混合感染患者在性别、年龄构成和疾病严重程度方面的差异。结果在部分COVID-19患者样本中检出副流感病毒、人偏肺病毒、冠状病毒NL63型和肺炎支原体,部分家庭成员样本中检出呼吸道合胞病毒、人偏肺病毒和甲型H3型流感病毒。混合感染与非混合感染患者在性别、年龄构成和疾病严重程度上的差异无统计学意义。结论海淀区COVID-19患者存在多种呼吸道病原体与SARS-CoV-2混合感染现象,且与家庭成员呼吸道样本的病原谱不同。混合感染没有加重COVID-19患者的症状。通过呼吸道病原体多重PCR检测,可提供更为精准的医疗策略。
Objective To analyze the infection status of common respiratory pathogens in coronavirus disease 2019(COVID-19)patients and their family members in Haidian district of Beijing and compare the disease severity between the COVID-19 patients with co-infections and the COVID-19 patients only infected with SARS-CoV-2.Methods Multiplex PCR was used to detect the pathogen spectrum by using the respiratory tract samples of COVID-19 patients and their family members collected by the Haidian Center for Disease Control and Prevention during January-June,2020.The differences in age and gender distributions and the severity of illness between the co-infection group and the non-co-infection group were compared.Results Parainfluenza virus,human metapneumovirus,NL63 coronavirus and Mycoplasma pneumoniae were detected in some samples of the COVID-19 patients,and respiratory syncytial virus,human metapneumovirus and H3 influenza virus were detected in samples of some family members.There was no statistical differences between the coinfection group and the non-co-infection group in gender,age distributions and the severity of illness Conclusion Coinfections of SARS-CoV-2 and other respiratory pathogens were observed in the COVID-19 patients in Haidian district.There was a difference in pathogen spectrum detected in samples between the COVID-19 patients and their family members.No increased morbidity or mortality were observed in the COVID-19 patients co-infected with other respiratory pathogens.Application of multiplex PCR can provide more accurate evidence for the development of medical strategies.
作者
刘方遥
张恒
焦莉洁
刘锋
Liu Fangyao;Zhang Heng;Jiao Lijie;Liu Feng(Haidian District Center for Disease Control and Prevention,Beijing 100094,China)
出处
《疾病监测》
CAS
CSCD
北大核心
2021年第4期355-359,共5页
Disease Surveillance
基金
国家科技重大专项(No.2017ZX10103004-002)。
关键词
新型冠状病毒肺炎
新型冠状病毒
多重PCR
病原谱
混合感染
Coronavirus disease 2019
Severe acute respiratory syndrome coronavirus 2
Multiplex PCR
Pathogen spectrum
Co-infection
