摘要
本研究以大豆为材料,采用盆栽和田间试验,探讨丛枝菌根真菌(AMF)对豆科作物生长的影响。盆栽试验设置了接种(+AMF)和不接种(-AMF)丛枝菌根真菌处理,田间试验设置了AMF菌丝非限制与限制处理。盆栽试验结果表明:接种AMF显著提高了大豆地上部生物量(16.5%)和大豆根瘤数(131.4%),地上部磷含量、磷吸收量、氮含量和氮吸收量也显著增加。田间试验中,AMF菌丝非限制处理下大豆的地上部生物量、根系生物量、根瘤数量分别比限制处理下显著提高了123.6%、61.5%和212.5%,地上部和根系磷吸收量、氮含量、氮吸收量均显著高于限制处理,大豆根际土壤速效氮和有效磷含量也均显著高于限制处理。本研究可为进一步认识豆科作物与AMF的共生关系及田间磷肥高效利用提供理论参考。
To explore the effects of arbuscular mycorrhizal fungi(AMF) on the growth of legume crop, pot and field experiments with soybean were conducted. Treatments of inoculation(+AMF) and non-inoculation with AMF(-AMF) were set up for the pot experiment, and AMF mycelium non-limited and limited for the field experiment. Results of the pot experiment showed that inoculation with AMF significantly increased soybean aboveground biomass(16.5%) and root nodules number(131.4%), above-ground plant phosphorus and nitrogen concentrations and uptakes. In the field trial, the above-ground and root biomasses and root nodules number under AMF mycelium non-limited were significantly increased by 123.6%, 61.5%, and 212.5% compared with those under the limited condition, respectively. Plant phosphorus uptake, nitrogen concentration and uptake, and soil available nitrogen and phosphorus concentrations were significantly higher under AMF mycelium non-limited than the limited both in both shoot and root. Our findings provide theoretical reference for further understanding the relationship between legume crop and AMF, as well as the efficient utilization of phosphorus fertilizer.
作者
刘云龙
钱浩宇
张鑫
郑成岩
邓艾兴
江瑜
张卫建
LIU Yun-long;QIAN Hao-yu;ZHANG Xin;ZHENG Cheng-yan;DENG Ai-xing;JIANG Yu;ZHANG Wei-jian(Institute of Crop Sciences,Chinese Academy of Agricultural Sciences/Key Laboratory of Crop Physiology and Ecology,Ministry of Agriculture and Rural Affairs,Beijing 100081,China;Jiangsu Collaborative Innovation Center for Modern Crop Production,Nanjing Agricultural University,Nanjing 210095,China)
出处
《应用生态学报》
CAS
CSCD
北大核心
2021年第5期1761-1767,共7页
Chinese Journal of Applied Ecology
基金
现代农业产业技术体系建设专项(绿肥,CARS-22)资助。
关键词
丛枝菌根真菌
大豆
磷
氮
生物固氮
养分利用效率
arbuscular mycorrhizal fungi
soybean
phosphorus
nitrogen
nitrogen bio-fixation
nutrient use efficiency
