摘要
碱性蛋白酶是工业上应用广泛的一类重要的蛋白酶制剂。目前国内高端洗涤用碱性蛋白酶主要依赖于进口,制约国内高端洗涤用蛋白酶的其中一个因素就是酶活性不高。从微生物菌株改造、蛋白质工程,以及通过优化启动子或信号肽的方式调控碱性蛋白酶的表达等3个方面阐述了提高微生物碱性蛋白酶酶活性的策略,最后对未来如何提高微生物来源的碱性蛋白酶活性的前景做了展望。
Alkaline protease is an important protease widely used in industry.At present,the domestic high-end washing protease mainly depends on the import,one of the factors restricting the domestic high-end washing protease is that the enzyme activity is not high.In this paper,the strategies to improve the activity of alkaline protease in microorganisms were described from three aspects:strain modification,protein engineering,and regulation of the expression of alkaline protease by optimizing the promoter or signal peptide.Finally,how to improve the activity of alkaline protease from microorganism in the future is prospected.
作者
袁媛
王蕾
石亚伟
YUAN Yuan;WANG Lei;SHI Ya-wei(Shanxi University,Institute of Biotechnology,Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education,Taiyuan 030006)
出处
《生物技术通报》
CAS
CSCD
北大核心
2021年第5期231-236,共6页
Biotechnology Bulletin
基金
山西省“1331”工程项目(PT201812)。
关键词
碱性蛋白酶
酶活力
策略
alkaline protease
enzyme activity
strategies
