摘要
目的对金标法在肺炎衣原体抗体检测中的临床效果进行分析。方法选取该院2018年1月—2019年1月收治的260例疑似肺炎衣原体感染患者,收集患者的血清标本,分别采用酶联免疫法、金标法与间接荧光法对患者的血清肺炎衣原体IgM抗体进行检测,并对其检测结果进行对比分析。结果 260例检测样本,采用间接荧光法进行检测显示,阳性标本136例,阳性率为52.3%;阴性标本124例,阴性率为47.7%。以间接荧光法检测结果作为金标准,对酶联免疫检测法与金标法的检测结果进行对比显示,金标法检测的阳性121例(92.4%)、阴性15例(11.6%),合计总符合168例(64.6%)。酶联免疫法检测的阳性46例(95.8%)、阴性90例(42.5%),总符合235例(90.4%),二者对比差异有统计学意义(P<0.05)。结论金标法进行肺炎衣原体抗体检验操作方法简单,且成本低廉、阳性符合率较高,但由于假阴性率也比较高,更适用于大批量的临床早期筛查与流行病学调查检验。
Objective To analyze the clinical effect of the gold labeling method in the detection of Chlamydia pneumoniae antibodies.Methods Selected 260 patients with suspected Chlamydia pneumoniae infection admitted in the hospital from January 2018 to January 2019.Serum samples of the patients were collected.Enzyme-linked immunosorbent assay,gold labeling method and indirect fluorescence method were used to detect the serum Chlamydia pneumoniae IgM antibody was tested and the test results were compared and analyzed.Results 260 samples were tested by indirect fluorescence method.136 cases were positive samples,the positive rate was 52.3%;124 cases were negative samples,and the negative rate was 47.7%.Using the indirect fluorescence method as the gold standard,the comparison of the results of the enzyme-linked immunoassay and the gold standard method showed that the gold standard method was positive 121 cases(92.4%),negative 15 cases(11.6%),and the total coincidence rate was 168 cases(64.6%),positive 46 cases(95.8%),negative 90 cases(42.5%),and total coincidence 235 cases(90.4%)by enzyme-linked immunosorbent assay.The difference between the two was statistically significant(P<0.05).Conclusion The gold labeling method for Chlamydia pneumoniae antibody testing is simple,low-cost,and has a high positive coincidence rate.However,due to the high false-negative rate,it is more suitable for large-scale early clinical screening and epidemiological investigations.
作者
余天会
YU Tianhui(Department of Laboratory Medicine,The Second People's Hospital of Baoshan City,Baoshan,Yunnan Province,678000 China)
出处
《世界复合医学》
2021年第4期82-84,共3页
World Journal of Complex Medicine
关键词
金标法
肺炎衣原体
抗体检测
临床效果
分析
Gold labeling method
Chlamydia pneumoniae
Antibody detection
Clinical effect
Analysis
