摘要
目的探讨MTHFR基因C677T及A1298C多态性与血浆同型半胱氨酸在复发性流产中的相关性。方法选取2016年12月—2019年6月江苏省淮安市第一人民医院收治的复发性流产女性100例为复发性流产组,选取同期100名有1次生育史但无不良孕产史女性作为对照组。采用限制性片段长度多态性检测MTHFR基因C677T和A1298C基因型;分析不同基因型复发性流产的发生风险;采用比色法检测同型半胱氨酸水平;比较不同基因型同型半胱氨酸水平差异。结果两组C677T和A1298C基因型比较,差异均有统计学意义(均P<0.05)。与C677T CC型比较,C677T TT型发生复发性流产的风险增加3.357倍(P=0.008);与A1298C AA型比较,A1298C CC型发生复发性流产的风险增加3.721倍(P=0.012)。复发性流产组同型半胱氨酸水平高于对照组,差异有统计学意义(P<0.05)。C677T TT型同型半胱氨酸水平高于C677T CC型和C677T CT型,差异均有统计学意义(均P<0.05);A1298C CC型同型半胱氨酸水平高于A1298C AA型和A1298C AC型,差异均有统计学意义(均P<0.05)。结论MTHFR基因C677T TT型、A1298C CC型复发性流产发生风险增高,且C677T及A1298C多态性可导致同型半胱氨酸水平升高。MTHFR基因C677T及A1298C突变可能通过改变同型半胱氨酸水平参与复发性流产发病。
Objective To investigate the correlation between MTHFR gene C677T and A1298C polymorphisms and plasma homocysteine in recurrent abortion.Methods A total of 100 women with recurrent abortion admitted to Huai’an First People’s Hospital,Jiangsu Province were selected as the recurrent abortion group,and 100 women with a history of one childbirth without history of adverse pregnancy were selected as the control group from December 2016 to June 2019.Restriction fragment length polymorphisms were used to detect the MTHFR gene C677T and A1298C genotypes;the occurrence risk of recurrent abortion with different genotypes was analyzed;colorimetric method was used to detect homocysteine level;the differences in homocysteine level between different genotypes were compared.Results There were statistically significant differences in the genotypes of C677T and A1298C between the two groups(all P<0.05).Compared with C677T CC type,C677T TT type had a 3.375-fold increased risk of recurrent abortion(P=0.008);Compared with A1298C AA type,A1298C CC had a 3.721-fold increased risk of recurrent abortion(P=0.012).The homocysteine level in the recurrent abortion group was higher than that in the control group,and the difference was statistically significant(P<0.05).Homocysteine level of C677T TT type was higher than that C677T CC type and C677T CT type,and the difference was statistically significant(P<0.05);homocysteine level of A1298C CC type was higher than that of A1298C AA type and A1298C AC type,and the difference was statistically significant(P<0.05).Conclusion The MTHFR gene C677T TT type and A1298C CC type have a higher risk of recurrent abortion,and C677T and A1298C polymorphisms can lead to elevated homocysteine level.The MTHFR gene C677T and A1298C mutations may be involved in the pathogenesis of recurrent abortion by changing homocysteine level.
作者
赵爱
张丽
孟琳
ZHAO Ai;ZHANG Li;MENG Lin(Department of Obstetrics,Huai’an First People’s Hospital,Jiangsu Province,Huai’an 223300,China;Department of Obstetrics,Affiliated Hospital of Xuzhou Medical University,Jiangsu Province,Xuzhou 221000,China)
出处
《中国医药导报》
CAS
2021年第15期117-120,共4页
China Medical Herald
基金
国家自然科学基金资助项目(81571437)。
