摘要
目的:探讨丹酚酸A(salvianolic acid A,SAA)通过调控过敏毒素C3a受体(anaphylatoxin C3a receptor,C3aR)的表达治疗缺血性疾病的作用机制。方法:构建体外血小板活化模型和中性粒细胞活化模型,采用比浊法检测血小板聚集率;流式细胞术检测血小板P选择素(P-selectin,CD62p)的表达;免疫荧光检测血小板在纤维蛋白原上的黏附;酶联免疫吸附测定法检测弹性蛋白酶(elastase,NE)、髓过氧化物酶(myeloperoxidase,MPO)、基质金属蛋白酶9(matrix metalloproteinase 9,MMP9)和乳铁蛋白(lactoferrin,LF)的水平。结果:与模型组对比,不同浓度的SAA能抑制二磷酸腺苷(adenosine diphosphate,ADP)诱导的血小板聚集、黏附、释放(P<0.05),显著抑制ADP诱导的C3aR的表达(P<0.05);与模型组对比,SAA能抑制中性粒细胞中NE、MPO、MMP9、LF的水平(P<0.05);C3aR抑制剂可抑制缺氧诱导的中性粒细胞脱颗粒(P<0.05)。结论:SAA可能通过调控C3aR的表达抑制血小板活化、聚集、黏附及中性粒细胞脱颗粒治疗临床缺血性疾病。
Objective:To investigate the mechanism of salvianolic acid A(SAA)in the treatment of ischemic diseases by regulating the expression of anaphylatoxin C3a receptor(C3aR).Methods:The in vitro platelet activation model and the neutrophil activation model were constructed,and platelet aggregation rate were detected by turbidimetry.Flow cytometry was used to detect the expression level of platelet P-selectin(CD62p).The adhesion ability of the platelet to fibrinogen was detected by immunofluorescence.ELISA was used to detected the levels of elastase(NE),myeloperoxidase(MPO),matrix metalloprotein(MMP9)and lactoferrin(LF).Results:Compared with the model group,SAA at different concentrations can inhibit the platelet aggregation,adhesion and release induced by adenosine diphosphate(ADP)(P<0.05),and significantly inhibit the expression level of C3aR induced by ADP(P<0.05).Compared with the model group,SAA can inhibit the levels of NE,MPO,MMP9 and LF in neutrophils(P<0.05).C3aR inhibitors can inhibit neutrophil degranulation induced by hypoxia(P<0.05).Conclusion:SAA may inhibit platelet activation,aggregation,adhesion and neutrophil degranulation to treat clinical ischemic diseases by regulating the expression level of C3aR.
作者
马璐璐
陈璐
张璐莎
方乐玉
李春晓
张丽媛
杨文杰
王倩怡
王虹
MA Lulu;CHEN Lu;ZHANG Lusha;FANG Leyu;LI Chunxiao;ZHANG Liyuan;YANG Wenjie;WANG Qianyi;WANG Hong(Tianjin University of Chinese Medicine,Tianjin China 301617)
出处
《中医学报》
CAS
2021年第6期1261-1266,共6页
Acta Chinese Medicine
基金
国家自然科学基金青年项目(81603329)
国家国际科技合作专项基金项目(2015DFA30430)。
关键词
丹参
丹酚酸A
血小板
中性粒细胞
C3aR
缺血性疾病
作用机制
salviae miltiorrhizae radix et rhizoma
salvianolic acid A
platelets
neutrophils
C3aR
ischemic disease
mechanism
