摘要
为了解铁皮石斛(Dendrobium officinale)WOX转录因子的功能,采用全基因组分析技术对铁皮石斛WOX家族成员进行鉴定,并进行生物信息学和表达模式分析。结果表明,铁皮石斛基因组中有9个WOX转录因子(DoWOX 1~DoWOX 9),大部分的DoWOXs含有2~3个外显子,启动子含有与激素诱导、逆境胁迫和生长发育有关的顺式作用元件。qPCR分析表明,DoWOX1、DoWOX2、DoWOX3、DoWOX4和DoWOX9在类原球茎中的表达量最高,DoWOX4在小花蕾中表达最高,随着花的发育,表达呈现下降趋势。此外,DoWOX3、DoWOX7和DoWOX9在合蕊柱上的表达量最高,而DoWOX4在唇瓣的表达量最高。因此,DoWOXs可能参与调控铁皮石斛的生长发育,且可能在维持类原球茎状态和花的发育中起重要作用。
In order to understand the function of transcription factor WOX in Dendrobium officinale,the WOX family members in D.officinale were identified by whole genome analysis techniques,the bioinformatics of WOXs and expression patterns of WOXs were analyzed.The results showed that there were nine WOX transcription factors in D.officinale genome,named DoWOX 1 to DoWOX 9,respectively.The most of DoWOXs contained 2-3 exons,while DoWOX9 had only one exon.The promoters of DoWOXs contained cis-regulatory elements related to hormone induction,stress responsive,plant growth and development.The expression of DoWOX1,DoWOX2,DoWOX3,DoWOX4 and Do WOX9 were the highest at protocorm-like body(PLB)stage by q PCR,which of DoWOX4 was the highest at small flower bud stage,and then decreased.In addition,the expressions of DoWOX3,DoWOX7 and Do WOX9 were the highest in columns,and that of DoWOX4 was the highest in lips.Therefore,DoWOXs might be involved in regulating the growth and development of D.officinale,and play an important role in maintenance of PLBs state and flower development.
作者
曾丹琦
张明泽
何春梅
王浩斌
俞振明
司灿
赵聪慧
李冬妹
段俊
ZENG Dan-qi;ZHANG Ming-ze;HE Chun-mei;WANG Hao-bin;YU Zhen-ming;SI Can;ZHAO Cong-hui;LI Dong-mei;DUAN Jun(Guangdong Provincial Key Laboratory of Applied Botany,Key Laboratory of South China Agricultural Plant Molecular Analysis and Gene Improvement,South China Botanical Garden,ChineseAcademy of Sciences,Guangzhou 510650,China;University of Chinese Academy of Sciences,Beijing 100049,China;Shunde Polytechnic,Foshan 528300,Guangdong,China)
出处
《热带亚热带植物学报》
CAS
CSCD
北大核心
2021年第3期301-310,共10页
Journal of Tropical and Subtropical Botany
基金
中国科学院华南农业植物分子分析与遗传改良重点实验室开放课题(KF202008)
广州市科技计划项目(201707010345)资助。
关键词
铁皮石斛
WOX转录因子
生物信息学
基因表达
qPCR
Dendrobium officinale
WOX transcription factor
Bioinformatics analysis
Gene expression
qPCR
