摘要
从贵州野生小白及植物根部分离并筛选出一株具有广谱抗病活性的内生菌,编号为GZFJ 015,毛壳属(Chaetomium sp.)真菌。为探明该试验菌株对香菇和灵芝在仿生态栽培中菌棒病原菌的抑制活性,采用生长速率法测定菌株GZFJ 015发酵液对不同食用菌病原菌菌丝生长的抑制率及室内毒力,并进一步研究温度和酸碱度对发酵液抗菌稳定性的影响。结果表明,内生菌GZFJ 015发酵液对香菇病原菌XG 1、XG 2、XG 3及灵芝病原菌LZ 1、LZ 2、LZ 3菌丝生长均具有抑制作用,其中LZ 2效果最显著(p<0.05),达85.1%,其余病原菌抑制率则在66.4%~83.8%范围内;发酵液毒力有效中浓度EC 50值依次为11.84、77.91、6.28、29.65、13.72、48.23μL·mL^(-1),对病原菌XG 3、LZ 1、XG 2和LZ 3毒力效果优于对照多菌灵。并且,发酵液的抑菌活性在80℃以下和pH值为6.0~11.0范围内相对稳定。
In this paper,an endophyte with broad-spectrum resistance to disease was isolated from the root of wild Bletilla striata in Guizhou province.It was designated GZFJ 015 and belonged to a fungus of the genus Chaetomium sp.In order to investigate the inhibitory activity of the tested strains against the pathogens from fungus rods of Lentinula edodes Ganoderma lucidum under the conditions of imitated ecological culture,the effects of fermentation liquid from strain GZFJ 015 on inhibitory rate and indoor virulence of mycelial growth of pathogens of different edible fungi was determined based on growth rate method.And,the effects of temperature and pH on the antibacterial stability of fermentation broth was further studied.The results showed that the fermentation broth from GZFJ 015 had inhibitory effects on the mycelial growth of L.edodes pathogens XG 1,XG 2,XG 3 and G.lucidum pathogens LZ 1,LZ 2 and LZ 3,and the inhibitory effect on LZ 2 was the most significant(p<0.05),up to 85.1%while the inhibition rates of other pathogens ranged from 66.4%to 83.8%.The effective medium concentration of fermentation broth(EC 50)was 11.84,77.91,6.28,29.65,13.72,48.23μL·mL^(-1),respectively.The toxicity to pathogens XG 3,LZ 1,XG 2 and LZ 3 was better than that of carbendazim.Moreover,the antimicrobial activity of the fermentation broth was relatively stable under 80℃and the pH was in the range of 6.0-11.0.
作者
曾茜
陈旭
杨雨
王晓敏
李玉平
ZENG Qian;CHEN Xu;YANG Yu;WANG Xiaomin;LI Yuping(School of Basic Medical,Guizhou University of Traditional Chinese Medicine,Guiyang 550025,China;Institute of Agricultural Resources and Environment,Guizhou Academy of Agricultural Sciences,Guiyang 550006,China;The Mushroom Research Institute,School of Agriculture,Guizhou University,Guiyang 550025,China)
出处
《种子》
北大核心
2021年第5期52-56,62,共6页
Seed
基金
贵州省科技计划项目“贵州中成药废弃物高效循环利用技术研究与集成示范”(黔科合支撑[2018]2781号)
“贵州灵芝药食两用生产关键技术研究”(黔科合支撑[2019]2773号)
“大宗食用菌适栽菌株选育”(黔科合重大专项字[2019]3007-1号)
“贵州特色副产物仿生态栽培灵芝关键技术研究与示范”(黔科合支撑[2016]2601号)
“贵州香菇产业化发展代料栽培新基质的研发与应用示范”(黔科合支撑[2016]2543号)。
关键词
内生菌
抗病活性
香菇和灵芝
病原菌
生防潜力
endophyte
anti-disease activity
Lentinula edodes and Ganoderma lingzhi
pathogens
biocontrol potential
