期刊文献+

毛细管超速离心技术在体外预测大量胎母输血综合征的研究 被引量:3

Study of Capillary Ultracentrifugation Technique on Prediction of Massive Maternal Transfusion Syndrome in Vitro
下载PDF
导出
摘要 目的探讨毛细管超速离心技术预测大量胎母输血综合征的临床运用,为ABO血型不一致的大量胎母输血提供一种更简便、快速的筛查方法。方法体外模拟胎母输血综合征的血液标本特点,按胎母输血综合征的出血量将O型育龄期非妊娠健康女性体检者EDTA抗凝血分别与A或B型新鲜脐带血按比例混合后室温下孵育1 h,用毛细管超速离心技术分离出远心端胎儿细胞,并进行ABO血型抗原鉴定;采用流式细胞术定量检测胎儿细胞百分比。结果胎母输血综合征出血量达到60 mL和100 mL时,毛细管超速离心技术敏感度、约登指数、阳性预测值和阴性预测值分别为0.8、0.75,0.75、0.7,0.941、0.9375,0.826、0.791。结论毛细管超速离心技术能简单、快速的识别ABO血型不一致的大量胎母输血综合征。 Objective To explore the clinical application of capillary ultracentrifugation in predicting massive maternal transfusion syndrome,which is more convenient to screen massive maternal transfusion syndrome with inconsistent blood types.Methods We mixed EDTA-anticoagulant blood of healthy women(type O on childbearing age)with type A/B umbilical cord blood for 1 hour,to simulate maternal transfusion syndrome.Fetal cells were isolated by capillary ultracentrifugation and performed blood group antigens identification.Furthermore,we performed flow cytometry to quantitatively verify the percentage of fetal red blood cells.Results When the fetal bleeding volume reached 60 and 100 mL,the results of sensitivity,Yoden index,positive predictive value and negative predictive value of capillary ultracentrifugation were respectively 0.8,0.75;0.75,0.7;0.941,0.9375 and 0.826,0.791.Conclusions Capillary ultracentrifugation technique can serve as effective method for massive maternal transfusion syndrome with inconsistent ABO blood group.
作者 张源秦 王美茹 徐璐 马怡然 杨融辉 郝一文 程大也 丛桂敏 ZHANG Yuan-qin;WANG Mei-ru;XU Lu(Department of Transfusion,The First Hospital of China Medical University 110000)
出处 《临床输血与检验》 CAS 2021年第3期301-305,共5页 Journal of Clinical Transfusion and Laboratory Medicine
基金 辽宁省自然科学基金计划重点项目(No.20180530022)资助。
关键词 大量胎母输血综合征 ABO血型不合 毛细管超速离心技术 Massive maternal transfusion syndrome ABO blood group incompatibility Capillary ultracentrifugation
  • 相关文献

参考文献3

二级参考文献40

  • 1Qureshi H,Massey E,Kirwan D,et al.BCSH guideline for the use of anti-D immunoglobulin for the prevention of haemolytic disease of the fetus and newborn.Transfus Med,2014,24(1):8-20
  • 2Lowe JB.Red cell membrane antigens.//Stamatoyannopoulos G,Majerus PW,Perlmutter RM,et al.The molecular basis of blood diseases.3rd ed.Beijing:Harcourt Asia Pte Ltd,2001:316.
  • 3British Committee for Standards in Haematology.Guidelines for the use of prophylactic anti-D immunoglobulin[OL].2006-09.[2014-04-08]http://www.bcshguidelines.com/documents/Anti-D-bcsh-07062006.pdf
  • 4National Institute for Health and Clinical Excellence(NICE).Routine antenatal anti-D prophylaxis for women who are rhesus D negative.(NICE TA156).2008-08[2014-04-08]http://www.nice.org[OL].uk/nicemedia/pdf/TA156Guidance.pdf.
  • 5British Committee for Standards in Haematology.Guidelines for the estimation of fetomaternal haemorrhage[OL].2009-09[2014-04-08]http://www.bcshguidelines.com/documents/BCSH_FMH_bcshsept2009.pdf.
  • 6British Committee for Standards in Haematology Guidelines for blood grouping and red cell antibody testing in pregnancy.Transfus Med,2007,17(4):252-262.
  • 7Milkins C,Berryman J,Cantwell C,et al.Guidelines for pre-transfusion compatibility procedures in blood transfusion laboratories.Transfus Med,2012,23(1):3-35.
  • 8Royal College of Obstetrics and Gynaecologists.The Use of Anti-D Immunoglobulin for Rhesus D Prophylaxis(RCOG Green Top Guideline 22)[OL]2011-03.[2014-04-08].http://www.rcog.org.uk/womens-health/clinical-guidance/use-anti-d-immunoglobulin-rh-prophylaxis-green-top-22.
  • 9Lee D,Contreras M,Robson SC,et al.Recommendations for the use of anti-D Ig immunoglobulin for Rh prophylaxis.Transfus Med,1999,9(1):93-97.
  • 10Royal College of Obstetrics and Gynaecologists.The management of gestational trophoblastic disease(RCOG Green Top Guideline 38)[OL].2010-02.[2014-04-08]http://www.rcog.org.uk/files/rcog-corp/GT38Management Gestational0210.pdf.

共引文献20

同被引文献16

引证文献3

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部