摘要
目的分析和研究三七药材微生物污染情况,探讨三七药材微生物污染的主要种类。方法参照2015年版《中国药典》四部通则1105、1106的方法,并结合传统生化和基质辅助激光解吸电离飞行时间质谱法(matrix assisted laser desorption ionization time-of-flight mass spectrometry,MALDI-TOF-MS)对纯化的菌落进行鉴定。最后,运用SPSS18.0软件对数据进行进一步地统计和分析。结果三七药材样品在经过100℃、30min热处理后,需氧菌总数呈现明显下降趋势,芽孢杆菌属、水生拉恩菌为三七药材污染概率较高的菌。结论在三七药材灭菌工艺的考察中,应该着重杀灭芽孢杆菌,为确保安全和药效,需要结合多种灭菌方法。
Objective To analyze and investigate the microbial pollution of Panax notoginseng,and discuss the main types of microbial pollution of Panax notoginseng.Methods According to the method of Chinese Pharmacopoeia(2015 edition),the purified colony was identified by combining traditional biochemistry method and matrix assisted laser desorption ionization time-of-flight mass spectrometry(MALDI-TOF-MS).Finally,SPSS 18.0 software was used for further statistics and analysis of the data.Results After heat treatment at 100℃for 30 min,the total number of aerobic bacteria in Panax notoginseng samples showed a significant downward trend,and Bacillus and Aquatic ranella were the bacteria with higher pollution probability.Conclusions In the investigation of the sterilization process of Panax notoginseng,Bacillus should be killed emphatically by combining a variety of sterilization methods,in order to ensure the safety and efficacy.
作者
白雯静
李志俊
周斌
徐雪梅
袁顺捷
BAI Wen-Jing;LI Zhi-Jun;ZHOU bin;XU Xue-Mei;YUAN Shun-Jie(Lanzhou Institute for Food and Drug Control,Lanzhou 730050,China)
出处
《食品安全质量检测学报》
CAS
北大核心
2021年第8期3405-3409,共5页
Journal of Food Safety and Quality
基金
甘肃省药品产业技术扶持项目(2019KF006)。
关键词
三七
基质辅助激光解吸电离飞行时间质谱法
微生物污染
Panax notoginseng
matrix assisted laser desorption ionization time-of-flight mass spectrometry
microbial contamination
