摘要
目的:基于近10年来文献研究中关于针刺治疗缺血性脑卒中的穴位及靶点研究,利用网络针灸学方法,探究针刺治疗缺血性卒中的有效穴位及生物学机制。方法:检索中国知网(CNKI)数据库、万方数据库和维普中文期刊数据库2009年7月—2019年7月针刺治疗缺血性卒中的相关实验研究文献,获得针刺选取穴位及其归经、有效治疗靶点,建立经脉-穴位-靶点数据库,运用cytoscape 3.6.1软件建立经脉-穴位-靶点网络模型,通过基因功能富集平台DAVID进行GO分析及KEGG富集通路分析。结果:共纳入文献437篇,涉及腧穴65个、靶点371个,筛选出18个关键靶点及8个有效穴位,得到8条主要富集结果及8个关键通路。结论:通过分析推测针刺可能通过调节相关关键靶点而影响TNF、NOD等信号通路,从而抑制炎症反应、减轻细胞凋亡等过程,达到治疗脑缺血的目的。
Objective:To explore the effectiveness and biological mechanism of acupuncture in treating ischemic stroke by using network acupuncture,based on the research of acupuncture points and targets of acupuncture in the treatment of ischemic stroke in the past 10 years.Methods:The related experimental research literature on acupuncture treatment of ischemic stroke was retrieved from the databases of CNKI,Wanfang,VIP from July 2009 to July 2019,it obtained acupuncture point selection and the meridian targets to establish the meridian-acupoint-target database,the meridian-acupoint-target network model was also established by using cytoscape 3.6.1 software,GO analysis and KEGG enrichment pathway analysis were performed through gene function enrichment platform DAVID.Results:A total of 437 articles were included,involving 65 acupoints and 371 targets.18 key targets and 8 effective points were selected,and 8 main enrichment results and 8 key pathways were obtained.Conclusion:Through analysis,it is speculated that acupuncture may affect signal pathways,such as signal pathways of TNF and NOD,by regulating relevant key targets,thereby inhibiting inflammatory response and reducing apoptosis and other processes to achieve the purpose of treating cerebral ischemia.
作者
谌爱华
蒋冰
王妙华
吴嘉萍
杨雨婷
贺福元
邓凯文
CHEN Aihua;JIANG Bing;WANG Miaohua;WU Jiaping;YANG Yuting;HE Fuyuan;DENG Kaiwen(Hunan University of Chinese Medicine, Changsha 410208, China;Hunan Provincial Key Laboratory of Drugability and Preparation Modification of Traditional Chinese Medicine, Changsha 410208, China;The First Affiliated Hospital of Hunan University of Chinese Medicine, Changsha 410007, China)
出处
《针灸临床杂志》
2021年第5期50-56,共7页
Journal of Clinical Acupuncture and Moxibustion
基金
国家自然科学基金,编号:81874507。
关键词
针刺
缺血性脑卒中
网络针灸学
Acupuncture
Ischemic stroke
Network acupuncture
