摘要
目的比较滤膜法、涂抹法和倾注法检测手术室内镜消毒效果的能力。方法随机选取该院2019年消毒后的手术室内镜230支为研究对象,同一受检内镜均分别接受滤膜法、涂抹法及倾注法检测,比较3种方法的检测合格率、病原菌检出率、菌落检出情况;用3种方法检测50支手术室内镜,计算检测的经济成本与时间成本。利用调查问卷对36所医院手术室内镜消毒效果检测方法的选择及影响因素进行调查。结果滤膜法检测各类型手术室内镜消毒效果的检测合格率明显低于涂抹法及倾注法,病原菌检出率明显高于涂抹法及倾注法,差异有统计学意义(P<0.05)。滤膜法检出菌落数>0~20、>20~100、>100~500、>500 CFU的内镜数明显多于涂抹法及倾注法,差异有统计学意义(P<0.05)。滤膜法的经济成本及时间成本高于涂抹法及倾注法。36所医院中,滤膜法的使用率明显低于涂抹法及倾注法,差异有统计学意义(P<0.05)。在手术室内镜消毒效果检测方法选择的影响因素中,36所医院对经济成本的选择率明显高于检测合格率及时间成本,差异有统计学意义(P<0.05)。结论涂抹法及倾注法检测手术室内镜消毒效果的能力较滤膜法差,建议各级医院在充分考量成本、效益及远期影响后,在条件允许的情况下尽量积极采用滤膜法完成手术室内镜消毒效果的检测。
Objective To compare the ability of filter membrane method,smear method and pouring method in the disinfection effect detection of operating room endoscope.Methods A total of 230 operating room endoscopes after disinfection in the hospital in 2019 were randomly selected as the research objects,the same endoscope was detected by filter membrane method,smear method and pouring method,compared the detection qualified rate,pathogenic bacteria detection rate,colony detection situation among the 3 methods.Fifty operating room endoscopes were detected by 3 methods,and evaluated the economic cost and time cost.The questionnaire was used to investigate the selection and influencing factors of the disinfection effect detection of operating room endoscope in 36 hospitals.Results The detection qualified rate of filter membrane method was significantly lower than that of smear method and pouring method,and the pathogenic bacteria detection rate was significantly higher than that of smear method and pouring method,the difference was statistically significant(P<0.05).The number of endoscopic colonies detected by the filter membrane method>0-20,>20-100,>100-500,>500 CFU was significantly more than that of the smear method and the pouring method,and the difference was statistically significant(P<0.05).The economic cost and time cost of filter membrane method were higher than those of smear method and pouring method.In 36 hospitals,the use rate of the filter membrane method was significantly lower than that of the smear method and the pouring method,and the difference was statistically significant(P<0.05).Conclusion The ability of smear method and pouring method to detect the disinfection effect of the operating room endoscope is worse than that of the filter membrane method.It is suggested that hospitals at all levels should actively use the filter membrane method to detect the disinfection effect of the operating room endoscope when the conditions permit after considering the cost,benefit and long-term influence.
作者
邢嘉翌
尹元
王佳
XING Jiayi;YIN Yuan;WANG Jia(Department of Clinical Laboratory,Shanghai Hospital of Integrated Traditional Chinese and Western Medicine,Shanghai University of Traditional Chinese Medicine,Shanghai 200082,China)
出处
《国际检验医学杂志》
CAS
2021年第12期1485-1488,1494,共5页
International Journal of Laboratory Medicine
关键词
手术室内镜
消毒效果
滤膜法
涂抹法
倾注法
operating room endoscope
disinfection effect
filter membrane method
smear method
pouring method
