摘要
目的观察健脾清化汤对甲硝唑耐药幽门螺杆菌(Hp)体外生长的影响及菌体内甲硝唑含量的变化,探讨健脾清化汤发挥作用的可能机制。方法选取甲硝唑耐药Hp临床菌株。实验组利用1、1/2、1/4、1/8、1/16、1/32倍健脾清化汤对Hp的生长及活动进行干预,对照组利用生理盐水进行对比,通过琼脂稀释法及Elisa法观察经2组药物作用后甲硝唑耐药Hp体外的生长情况及菌体内甲硝唑含量的变化。结果体外抑菌试验显示,1、1/2、1/4倍健脾清化汤对甲硝唑耐药Hp的生长具有抑制作用,其最低抑菌浓度为1/4倍浓度;Elisa法结果显示,较对照组,经健脾清化汤作用后的各组耐药Hp内甲硝唑含量均显著上升,呈梯度变化,各组之间差异有统计学意义(P<0.05)。结论1、1/2、1/4倍健脾清化汤的能够抑制甲硝唑耐药Hp的生长,同时不同浓度健脾清化汤均能改变菌体内甲硝唑含量,其机制可能与影响Hp外排泵有关。
Objective To observe the effect of Jianpi Qinghua Decoction on the growth of metronidazole resistant Helicobacter pylori(HP)in vitro and the change of metronidazole content in vivo,and to explore the possible mechanism of Jianpi Qinghua Decoction.Methods Clinical strains of metronidazole resistant HP were selected.In experimental group,1,1/2,1/4,1/8,1/16,1/32 times of Jianpi Qinghua Decoction was used to intervene the growth and activity of HP.In control group,normal saline was used for comparison.The growth of metronidazole resistant HP in vitro and the change of metronidazole content in bacteria were observed by agar dilution method and ELISA method.Results Antibacterial test in vitro showed that 1,1/2,1/4 times of Jianpi Qinghua Decoction had inhibitory effect on the growth of metronidazole resistant HP,and its minimum inhibitory concentration was 1/4 times of concentration;ELISA Results showed that Jianpi Qinghua Decoction was more effective compared with the control group.The contents of metronidazole in drug-resistant HP in each group were increased significantly,showing a gradient change,and the difference between each group was statistically significant(P<0.05).Conclusion 1,1/2,1/4 times of Jianpi Qinghua Decoction can inhibit the growth of metronidazole resistant HP.At the same time,different concentrations of Jianpi Qinghua Decoction can change the content of metronidazole in bacteria.The mechanism may be related to the influence of HP efflux pump.
作者
董礼刚
张会存
姬莉
李舒敏
刘婉琼
尤佳
宋瑶
汪红兵
DONG Li-gang;ZHANG Hui-cun;JI Li;LI Shu-min;LIU Wan-qiong;YOU Jia;SONG Yao;WANG Hong-bing(Digestion Center,Beijing Hospital of Traditional Chinese Medicine Affiliated to the Capital Medical University,Beijing 100010,China;Beijing Institute of Traditional Chinese Medicine,Beijing 100010)
出处
《北京中医药》
2021年第4期374-377,共4页
Beijing Journal of Traditional Chinese Medicine
基金
国家自然科学基金面上项目(8177150984)
北京市自然科学基金面上项目(7172098)。
关键词
健脾清化汤
甲硝唑
耐药
幽门螺杆菌
体外生长
Jianpi Qinghua Decoction
metronidazole
drug resistance
helicobacter pylori
growth in vitro
