摘要
根据NCBI已公布的花斑无须鲶全基因组序列,利用MISA软件对花斑无须鲶全基因组的6种完整型微卫星进行筛选并分析其分布特征.结果如下:在花斑无须鲶全基因组(约1.03Gb)中符合条件的微卫星序列共336037个,丰度为326个/Mb.微卫星总长度为7720686 bp,占花斑无须鲶全基因组的0.75%.其中二碱基类型的微卫星数目最多,为145318个,占微卫星总数的43.24%,其次分别为单碱基(37.12%)、三碱基(11.00%)、四碱基(7.39%)、五碱基(1.04%)和六碱基(0.21%).花斑无须鲶全基因组微卫星中的优势碱基类别按照数量从高到低排列依次为A、AC、AG、AT、AAT、AAAT、TATC、AAG、AAC和TGA,共有305243个,占微卫星总数的90.84%,A、T碱基在微卫星中占绝对优势.
According to the complete genome sequence of Ageneiosus marmoratus published on NCBI,the microsatellites of the whole genome of Ageneiosus marmoratus are screened and analyzed by using MISA software.The results are as follows:there are 336037 microsatellite sequences in the whole genome of Ageneiosus marmoratus(about 1.03 Gb),and the abundance is 326/MB.The total length of microsatellites is 7720686 bp,accounting for 0.75%of the whole genome.Among them,the number of microsatellites with two bases is the most,which is 145318,accounting for 43.24%of the total number of microsatellites,followed by single base(37.12%),triple base(11.00%),four base(7.39%),five base(1.04%)and six base(0.21%).The dominant base types of microsatellites in the whole genome of Ageneiosus marmoratus are A,AC,AG,AT,AAT,AAAT,TATC,AAG,AAC and TGA,with a total of 305243,accounting for 90.84%of the total number of microsatellites.
作者
苏孟园
杨汶珊
唐荣叶
徐杰杰
王涛
尹绍武
Su Mengyuan;Yang Wenshan;Tang Rongye;Xu Jiejie;Wang Tao;Yin Shaowu(School of Marine Science and Engineering,Nanjing Normal University,Nanjing 210023,China;Jiangsu Province Engineering Research Center for Aquati Animals Breeding and Green Efficient Aquacultural Technology,Nanjing 210023,China)
出处
《南京师范大学学报（工程技术版）》
CAS
2021年第2期65-71,共7页
Journal of Nanjing Normal University(Engineering and Technology Edition)
基金
江苏省农业重大新品种创制项目(PZCZ201742)
江苏省重点研发计划(现代农业)重点项目(BE2017377)
江苏省农业科技自主创新资金项目(CX(19)2034)
南京师范大学大学生创新创业训练计划项目。
关键词
花斑无须鲶
微卫星
基因组
分布特征
Ageneiosus marmoratus
microsatellite
genome
distribution characteristics
