摘要
为研究三峡库区中段水体微生物多样性的变化以及群落结构与环境理化性质的相关性,以三峡库区涪陵、忠县、万州为研究区域,分别于2018年高水位期(1、3月)和低水位期(5、7、9月)进行采样分析,结合水质分析及16S rRNA高通量测序分析,对中段水体3个点位的微生物多样性进行研究,并对不同时期的微生物群落结构与环境因子进行相关分析。结果发现:三峡库区中段水体微生物多样性表现为库区中段上游涪陵高于中下游忠县和万州。经群落组成分析得出,库区中段水体微生物的优势菌门无差异,主要优势菌群为Proteobacteria(80.50%~21.51%)、 Actinobacteria (32.60%~9.98%)、 Bacteroidetes (6.89%~3.50%)、 Cyanobacteria(9.65%~0.08%)等。微生物群落结构组成存在显著的时间变化性,Actinobacteria、 Firmicutes、Planctomycetes与Cyanobacteria等菌门的相对丰度在库区高水位期高于低水位期,而最优势菌门Proteobacteria的相对丰度在高水位期低于低水位期。冗余分析表明总氮、溶解氧、水位是影响三峡水库中段水体微生物群落结构的主要环境因子。
In order to study the changes of microbial diversity and the correlation between microbial community structure and environmental properties in different periods of time, Fuling County, Zhongxian County and Wanzhou County,the middle section of the Three Gorges Reservoir area,were chosen as the research area. The water quality analysis and 16S rRNA high-throughput sequencing was used to study the microbial diversity of the sampling points during the high water period (January, March) and the low water period (May, July,September) in 2018,and the correlation between microbial community structure and environmental factors in different periods was analyzed. Results showed that the microbial diversity of Fuling in the upper reaches of the middle section of the Three Gorges Reservoir was higher than that of Zhongxian and Wanzhou in the middle and lower reaches. According to the analysis of community composition,the dominant bacteria in the middle section of the reservoir area did not differ significantly at the phylum level. The main dominant bacteria were Proteobacteria (80. 50% ~ 21. 51%),Actinobacteria (32. 60% ~ 9. 98%),Bacteroidetes (6. 89% ~ 3. 50%),Cyanobacteria (9. 65% ~ 0. 08%),etc. The composition of the microbial community has significant temporal variability,for example,the relative abundance of Actinobacteria,Firmicutes,Planctomycetes and Cyanobacteria was higher in the high water period than in the low water period,while the relative abundance of Proteobacteria was lower in the high water period than in the low water period. Redundancy analysis indicated that total nitrogen,dissolved oxygen and water level were the main environmental factors affecting microbial community structure in the middle section of the Three Gorges Reservoir.
作者
秦宇
郑望
张曦
蒋成勇
QIN Yu;ZHENG Wang;ZHANG Xi;JIANG Cheng-yong(Key Laboratory of Hydraulic and Waterway Engineering of the Ministry of Education,Chongqing Jiaotong University,Chongqing 400074,China)
出处
《长江流域资源与环境》
CAS
CSSCI
CSCD
北大核心
2021年第5期1184-1193,共10页
Resources and Environment in the Yangtze Basin
基金
国家自然科学基金项目(51609026)
重庆市教委科学技术研究项目(KJ1500506)
重庆市基础科学与前沿技术研究项目(cstc2017jcyjAX0280)
重庆市研究生科研创新基金项目(CYS19246)。
关键词
微生物多样性
环境因子
冗余分析
三峡库区
群落结构
microbial diversity
environmental properties
redundancy analysis
Three Gorges Reservoir
community structure
