摘要
本文采用高效液相色谱-柱后衍生法对变性淀粉中的黄曲霉毒素G_(2)、G_(1)、B_(2)和B_(1)含量进行测定。样品采用乙腈-水(84∶16,v/v)提取,经免疫亲和柱净化,C18色谱柱分离,以乙腈-甲醇-水(10∶30∶60,v/v)为流动相进行洗脱,用HPLC光化学衍生-荧光检测器检测样品含量。结果表明,4种黄曲霉毒素的标准曲线线性均大于0.9993,回收率96%~104%,黄曲霉G_(2)、G_(1)、B_(2)和B_(1)的检出限分别为0.0100μg/kg、0.0300μg/kg、0.0100μg/kg和0.0300μg/kg。该方法操作简便,准确度高,适用于变性淀粉中黄曲霉毒素的测定。与柱前衍生法相比,该法能节省检测时间和检测成本,同时减少化学试剂对检测人员的伤害。
In this paper,the contents of aflatoxin G_(2)、G_(1)、B_(2)、B_(1) in modified starch were determined by high performance liquid chromatography(HPLC)and post-column photochemical reaction.The samples were extracted by acetonitrile-water(84∶16,v/v),purified by immunoaffinity column,separated by C_(18) column,eluted with acetonitrile-methanol-water(10∶30∶60,v/v)as mobile phase,and then the contents of samples were detected by post-column photochemical derivation-fluorescence detector.The results showed that the correlation coefficients of all aflatoxins were more than 0.9993,the recoveries were 96%~104%,and the detection limits of aflatoxin G_(2)、G_(1)、B_(2)、B_(1) were 0.0100μg/kg,0.0300μg/kg,0.0100μg/kg and 0.0300μg/kg,respectively.The method was simple,accurate and suitable for the determination of aflatoxin in modified starch.Compared with the pre-column derivatization method,this method could save the detection time and cost,and at the same time,reduce the harm of the chemical reagents to the testers.
作者
杨娟
YANG Juan(Shanghai Industrial Microbiology Institute Tech. Co, Ltd, Shanghai 200233)
出处
《工业微生物》
CAS
2021年第3期31-35,共5页
Industrial Microbiology
关键词
黄曲霉毒素
高效液相色谱法
免疫亲和柱
荧光检测器
aflatoxin
high performance liquid chromatography
immunoaffinity column
fluorescence detector
