摘要
建立了离子交换固相萃取-反相高效液相色谱法同时测定黄酒中游离鸟嘌呤、次黄嘌呤、黄嘌呤和腺嘌呤的分析方法,并对15批次酒样中4种游离嘌呤进行了测定。样品煮沸除去乙醇后,经MCX混合阳离子交换固相萃取柱净化后,可用高效液相色谱进行分析检测。结果表明,在0.1~25 mg/L质量浓度范围内,鸟嘌呤、次黄嘌呤、黄嘌呤和腺嘌呤的标准曲线线性良好(R>0.9999),4种嘌呤检出限分别为0.1、0.1、0.2、0.1 mg/L,回收率为83.13%~98.32%,4种分析物的日内和日间测定精密度均小于4.0%。此方法简便,快捷,可靠。
A high performance liquid chromatographic method was developed for the simultaneous determination of free guanine,hypoxanthine,xanthine and adenine in 15 Huangjiu(Chinese rice wine)samples.To reduce matrix interferences and increase method sensitivity,MCX mixed cation exchange solid phase extraction column was employed for sample clean-up.After the sample was boiled to remove ethanol,it was purified by MCX column and analyzed by high performance liquid chromatography.The standard curve of purines showed good linearity in the range of 0.1-25 mg/L,with the correlation coefficient R larger than 0.9999.The lowest detectable concentrations of guanine,hypoxanthine,xanthine and adenine were 0.1,0.1,0.2,0.1 mg/L,respectively.The recovery rates were between 83.13%-98.32%.The intra-day and inter-day assay precision(RSD)of these four purines were less than 4.0%.This method is simple,fast and reliable.
作者
刘镇
张瑛
郑云峰
胡翎榆
屠生辉
吴坚
LIU Zhen;ZHANG Ying;ZHENG Yunfeng;HU Lingyu;TU Shenghui;WU Jian(Shaoxing Testing Institute of Food and Drug,Shaoxing National Center for Quality Supervision&Testing of Rice Wine,Shaoxing 312000,China;Shaoxing Institute of Standardization,Shaoxing 312000,China)
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2021年第11期236-240,共5页
Food and Fermentation Industries
基金
浙江省食品药品监督管理局科技计划项目(2019005)。
关键词
嘌呤
黄酒
高效液相色谱
离子交换固相萃取
purine
Huangjiu
high performance liquid chromatography
ion exchange solid phase extraction
