摘要
为建立“阳光玫瑰”葡萄嫩茎组织培养体系,以其嫩茎作为外植体,进行灭菌时间的确定,初代培养基、继代培养基、生根培养基的筛选及抗褐化培养,以期为后续进行种苗脱毒及生产种植提供技术基础.结果表明:嫩茎用0.1%HgCl2灭菌9 min为最佳处理时间,萌芽率达到最大,外植体无污染无死亡;MS+1.0 mg/L KT+0.1 mg/L NAA是嫩芽茎段芽分化最适宜的培养基,萌芽率达93.3%,平均诱导2个芽;通过添加PVP有良好的抗褐化效果,以2.0 mg/L浓度为适宜;继代培养则筛选出MS+1.5 mg/L KT+0.1 mg/L IAA为无菌芽增殖分化较好的培养基;生根诱导以0.3 mg/L NAA为好.
In order to establish the tissue culture system of“Shine Muscat”grape,the culture medium,rooting medium and anti-browning culture,for providing a technical basis for the subsequent detoxification of seedlings and production.The results show that the best treatment time was sterilizated with 0.1%HgCl2 for 9 minutes,the germination rate reached the maximum,and the explants had no pollution and death;MS+1.0 mg/L KT+0.1 mg/L NAA was the most suitable medium for bud differentiation,with a germination rate of 93.3%,inducing 2 buds on average.By adding PVP,it has a good anti-browning effect with a concentration of 2.0 mg/L.MS+1.5 mg/L KT+0.1 mg/L IAA was suitable for sterile buds proliferation and differentiation;0.3 mg/L NAA was suitable for rooting induction.
作者
农艳丰
王利园
李健
NONG Yan-feng;WANG Li-yuan;LI Jian(College of Agricultural and Food Engineering, Baise University, Baise Guangxi 533000, China)
出处
《西南师范大学学报（自然科学版）》
CAS
2021年第6期52-56,共5页
Journal of Southwest China Normal University(Natural Science Edition)
基金
广西自然科学基金项目(2018GXNSFBA050026)
广西硕士学位授予单位立项建设授权点项目(桂学位[2018]7号).
