摘要
目的探讨茶多酚对重铬酸钾(PD)所致小鼠急性肾损伤的保护作用及其机制。方法将无特定病原体级断乳昆明小鼠分为对照组、模型组和茶多酚低、中、高剂量组,每组12只。每天上午对照组小鼠予0.9%氯化钠溶液灌胃,其余4组小鼠予剂量为4.275 mg/kg体质量的PD溶液灌胃;下午对照组和模型组小鼠均予0.9%氯化钠溶液灌胃,茶多酚低、中、高剂量组小鼠分别予200、400、600 mg/kg体质量的茶多酚溶液灌胃;每次灌胃体积为0.3 mL,每日1次,连续2周。实验期间称量小鼠体质量。实验结束处死小鼠,称量小鼠肾脏,计算肾脏脏器系数;采用两点法测定小鼠血清中尿素氮和肌酐水平,采用比色法检测小鼠血清中过氧化氢酶(CAT)和谷胱甘肽过氧化物酶(GSH-Px)活力;观察小鼠肾脏病理组织学改变情况。结果与对照组比较,模型组小鼠体质量下降(P<0.05),肾脏质量、肾脏脏器系数和血清中尿素氮、肌酐水平均升高(P值均<0.05),血清CAT和GSH-Px活力均下降(P值均<0.05)。与模型组比较,3个茶多酚剂量组小鼠体质量均升高(P值均<0.05),肾脏质量、肾脏器系数和血清中尿素氮、肌酐水平均下降(P值均<0.05),血清中CAT和GSH-Px活力均随茶多酚干预剂量的升高而升高(P值均<0.05)。模型组小鼠出现以肾小管损伤为主的急性肾损伤改变;3个茶多酚剂量组小鼠肾脏组织病变与模型组比较均有所好转,并呈剂量-效应关系。结论茶多酚对PD诱导的小鼠急性肾损伤具有保护作用,呈一定程度的剂量-效应关系;其作用机制与茶多酚可减轻或逆转肾脏中氧化应激和炎症反应有关。
Objective To explore the protective effect of tea polyphenols and its mechanism in potassium dichromate(PD)-induced acute renal injury in mice. Methods The specific pathogen free weaned Kunming mice were divided into control group, model group and low-, middle-and high-dose tea polyphenols groups, with 12 mice in each group. Mice in the control group were given 0.9% sodium chloride solution, and mice in other four groups were given PD solution with 4.275 mg/kg body weight every morning by intragastric administration. Then, mice in the control group and model group were given 0.9% sodium chloride solution in the afternoon, while mice in the low-, middle-and high-dose tea polyphenols groups were given 0.3 mL tea polyphenols solution with a dose of 200, 400 or 600 mg/kg body weight, respectively by gavage, once a day for two consecutive weeks. The body mass of mice was weighed during the experiment. At the end of the experiment, the mice were sacrificed. The kidneys were removed and weighed. The kidney organ coefficients were calculated. The levels of urea nitrogen and creatinine in serum were determined by two-point method, the activities of catalase(CAT) and glutathione peroxidase(GSH-Px) in serum of mice were detected by colorimetry. The pathological change of kidney in mice was observed. Results The body weight of mice in the model group decreased(P<0.05), while the kidney mass, renal organ coefficient, serum levels of urea nitrogen and creatinine increased(all P<0.05), and the serum activities of CAT and GSH-Px decreased(all P<0.05) compared with the control group. The body weight of mice in the three tea polyphenols groups increased(all P<0.05), while the kidney mass, renal organ coefficient, urea nitrogen and creatinine levels in serum decreased(all P<0.05), and the activities of CAT and GSH-Px in serum increased with the increasing intervention dose of tea polyphenols(all P<0.05) compared with the model group. The change of acute renal injury was mainly caused by renal tubular injury in the model group. The pathological changes of renal tissue in the three tea polyphenols intervention groups were improved compared to that in the model group, and the improvement showed a dose-effect relationship with the intervention of tea polyphenols. Conclusion Tea polyphenols have a protective effect on PD-induced acute renal injury with a dose-effect relationship. Its mechanism of action is related to the fact that tea polyphenols can reduce or reverse oxidative stress and inflammation in the kidney.
作者
唐元娟
刘燕群
彭敏
程露
甘维康
杨晔
李杜
鲁雄兵
TANG Yuan-juan;LIU Yan-qun;PENG Min;CHEN Lu;GAN Wei-kang;YANG Ye;LI Du;LU Xiong-bin(School of Medicine,Jianghan University,Wuhan,Hubei 430056,China)
出处
《中国职业医学》
CAS
北大核心
2021年第1期46-50,共5页
China Occupational Medicine
基金
湖北省高等学校优秀中青年科技创新团队项目(T201224)
2015年度“黄鹤英才(专项)计划”
江汉大学大学生科研项目(2017yb301)。
关键词
茶多酚
重铬酸钾
急性肾损伤
氧化应激
炎症反应
小鼠
Tea polyphenols
Potassium dichromate
Acute renal injury
Oxidative stress
Inflammatory reaction
Mouse
