摘要
采用生物信息学方法对茶树髓细胞增生蛋白(myelocytomatosisproteins,MYCs)基因家族成员的理化性质、进化关系、蛋白质结构、顺式作用元件进行分析,运用qRT-PCR技术研究MYC基因家族成员在茶树中的表达。结果表明:茶树MYC为不稳定蛋白、亲水蛋白、非分泌蛋白、非跨膜蛋白,均呈酸性;MYC基因家族6个成员都定位于细胞核中;基因家族二级结构主要以无规则卷曲和α-螺旋为主,均含1个外显子,无内含子,motif分布相似的成员,其三级结构也相似;茶树MYC基因和拟南芥的亲缘关系更近;茶树MYC家族基因启动子均主要含有光信号、茉莉酸甲酯和脱落酸等3个顺式作用元件。qRT-PCR分析显示,MYC家族成员主要在茶树的根和种子中表达,可能在茶树对硒吸收累积过程中发挥调控作用。
Physical and chemical properties,evolutionary relationship,protein structure and cis-acting elements of myelocytomatosis proteins(MYCs)gene family in tea plant were analyzed by bioinformatics methods,and qRT-PCR was used to study the expression patterns of MYC gene family in.The results showed that tea plant MYCs(CsMYC protein)were acidic,unstable,hydrophilic,non-secretory and non-transmembrane proteins.Subcellular localization predictions indicated that the 6 MYC genes were all in the nucleus.Gene structure and motif analysis showed that the secondary structures of MYC genes were mainly random coils andα-helix,and they all contained an exon and no introns.Moreover,members with similar motif distribution had similar tertiary structures.Evolutionary analysis showed that the CsMYC genes in tea plant were closer to AtMYC genes in Arabidopsis.Analysis of cis-acting elements suggested that the gene family mainly contained circadian,methyl jasmonate(MeJA)and abscisic acid(ABA).Results of qRT-PCR showed that the 6 CsMYC genes were mainly expressed in the roots and seeds,and might play important regulatory roles in the process of selenium absorption and accumulation in tea plant.
作者
曹丹
刘艳丽
马林龙
金孝芳
CAO Dan;LIU Yanli;MA Linlong;JIN Xiaofang(Fruit and Tea Research Institute,Hubei Academy of Agricultural Sciences,Wuhan Hubei 430064,China;Key Laboratory of Tea Science of Ministry of Education/National Research Center of Engineering and Technology for Utilization of Botanical Functional Ingredients/Co-Innovation Center of Education Ministry for Utilization of Botanical Functional Ingredients,Changsha,Hunan 410128,China)
出处
《湖南农业大学学报（自然科学版）》
CAS
CSCD
北大核心
2021年第3期291-298,共8页
Journal of Hunan Agricultural University(Natural Sciences)
基金
湖北省科学技术厅重点研发计划项目(2020BBA038)
湖北省财政厅农业科技创新中心项目(2019–620–000–001–24)
湖北省农业科学院青年基金项目(2018NKYJJ12)。
关键词
茶树
髓细胞增生蛋白基因家族
生物信息学
基因表达
硒
tea plant
myelocytomatosis protein gene family
bioinformatics
gene expression
selenium
