摘要
目的 基于网络药理学研究益气活血药对黄芪-川芎治疗缺血性脑卒中的作用机制。方法从TCMSP数据库筛选黄芪、川芎活性化合物及靶点,在Uniprot数据库转化为基因靶点,通过Cytoscape 3.8.0构建化合物-靶点网络。在OMIM、DisGeNET、TTD数据库及文献报道收集缺血性脑卒中的治疗靶点,构建疾病-靶点网络。将化合物-靶点网络与疾病-靶点网络结合,筛选黄芪-川芎药对治疗缺血性脑卒中潜在核心靶点。利用STRING平台,获得核心靶点PPI网络。将核心靶点进行GO功能富集分析及KEGG富集分析。结果筛选出黄芪-川芎药对的活性化合物17个,潜在核心靶点18个,包括IL-1β、NOS3、CASP3、BDNF等,可通过调节PI3K/AKT信号通路、MAPK信号通路、TLR信号通路、NF-κB信号通路等多个途径,参与调节NO生物合成、细胞凋亡、炎症及免疫反应、血管生成及器官再生等机制,从而改善缺血性脑卒中病理损伤。结论通过网络药理学揭示了益气活血药对黄芪-川芎"多组分、多靶点、多机制"治疗缺血性脑卒中的作用特点,为其深入研究提供了科学依据。
Objective To explore the mechanism of Hedysarum Multijugum Maxim.and Chuanxiong Rhizoma for ischemic stroke based on network pharmacology.Methods The active chemical components and targets of Hedysarum Multijugum Maxim.zand Chuanxiong Rhizoma were obtained from TCMSP database.Targets were converted to genes by Uniprot database.Components-targets network were established by Cytoscape 3.8.0.The targets of ischemic stroke were obtained from OMIM、DisGeNET、TTD database and literatures,which constituted disease-targets network.Components-targets network and disease-targets network were merged and the potential core targets were predicted.Potential core targets were entered into the STRING platform to obtain a protein-protein interaction(PPI)network.Finally,GO functional enrichment analysis and the KEGG enrichment analysis of potential core targets were calculated.Results There are 18 predicted core targets for 17 compounds and the core targets includined IL1B、NOS3、CASP3、BDNF,which may via regulating PI3K/AKT signaling pathway,MAPK signaling pathway,TLR signaling pathway,NF-κB signaling pathway,etc,affected regulations of NO biosynthesis,apoptosis,inflammation and immune response,angiogenesis and organ regeneration,etc,so as to participate in a series of physiological and pathological processes of ischemic stroke.Conclusion Network pharmacology exhibited multi-component,multi-target,and multi-mechanism biological effects of Hedysarum Multijugum Maxim and Chuanxiong Rhizoma,which provides a scientific basis for further study.
作者
陈红阳
杨志华
贾壮壮
刘震
郭利平
CHEN Hongyang;YANG Zhihua;JIA Zhuangzhuang;LIU Zhen;GUO Liping(Tianjin University of Traditional Chinese Medicine,Tianjin 301617,China;First Teaching Hospital of Tianjin University of TCM,Tianjin 300381,China;Baotou Central Hospital,Baotou 014040,China;Tianjin Academy of Traditional Chinese Medicine Affiliated Hospital,Tianjin 300120,China)
出处
《云南中医学院学报》
2021年第1期88-97,共10页
Journal of Yunnan University of Traditional Chinese Medicine
基金
天津市科技计划项目(15ZXLCSY00020)
内蒙古自治区自然基金项目(2020BS08012)。
关键词
网络药理学
黄芪-川芎
缺血性脑卒中
network pharmacology
Hedysarum Multijugum Maxim.and Chuanxiong Rhizoma
cerebral ischemic stroke
