摘要
目的探讨复方苦参注射液对大鼠急性出血重症性放射性肠炎的治疗作用。方法将24只SD大鼠随机分为空白组、模型组与治疗组。模型组和治疗组的大鼠予以4 mV X线单次30 Gy照射腹部建立急性出血重症放射性肠炎模型。空白组、模型组予以生理盐水腹腔注射,治疗组予复方苦参注射液(0.3 mL/100 g)腹腔注射。给药14 d后,观察各组大鼠的死亡等情况,并于照射后第15天处死剩余大鼠。对于肠道组织用光镜和电镜观察结构改变,免疫组化检测血管内皮生长因子(VEGF)表达,ELISA法检测白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)表达。结果复方苦参注射液能明显改善大鼠肠道黏膜组织以及细胞微结构的破坏,降低治疗大鼠血便和死亡率。治疗组大鼠的肠道炎症因子IL-6、TNF-α以及VEGF含量显著低于模型组,差异有统计学意义(P<0.05)。结论复方苦参注射液能治疗大鼠急性出血重症放射性肠炎,缓解炎症及出血,并能减少死亡率。
Objective:To investigate the therapeutic effect of Compound Kushen Injection on the acute severe hemorrhagic radiation enteritis in rats.Methods:A total of 24 SD rats were randomly divided into the blank group,model group and treatment group.The model group and treatment group were irradiated with 4 mV X rays at a single 30 Gy abdomen to establish an acute severe hemorrhagic radiation enteritis model.The blank group and the model group received intraperitoneal injection of normal saline,and the treatment group received intraperi⁃toneal injection of Compound Kushen Injection(0.3 mL/100 g).Treatment lasted for 14 days.The performance and death of rats in each group was recorded,and the remaining rats were sacrificed 15 days after irradiation.The structural changes of the intestinal tissues were observed with light microscope and electron microscope,the expres⁃sion of VEGF was detected by immunohistochemistry,and the expression of IL-6 and TNF-αwas detected by ELI⁃SA.Results:Compound Kushen Injection cuold significantly improve the destruction of intestinal mucosal tissue and cell microstructure in rats,and reduce the bloody stools and the mortality of the rats in the treatment group.The levels of inflammatory factors IL-6,TNF-αand VEGF in the intestinal tract of rats in the treatment group were significantly lower than those in the model group,and the difference was statistically significant(P<0.05).Conclusion:Compound Kushen Injection can treat the acute severe hemorrhagic radiation enteritis in rats,relieve inflammation and bleeding,and reduce mortality.
作者
许文婧
马玥诗
王佩
高丽萍
杨志祥
孟令占
Xu Wenjing;Ma Yueshi;Wang Pei;Gao Liping;Yang Zhixiang;Meng Lingzhan(Chongqing Traditional Chinese Medicine Hospital,Chongqing 400021,China)
出处
《中国中医急症》
2021年第6期959-962,共4页
Journal of Emergency in Traditional Chinese Medicine
基金
成都中医药大学“杏林学者”学科人才科研提升计划医院专项(YYZX2019061)
重庆市科研机构绩效激励引导专项项目基金(cstc2018jxj10194)。
