摘要
小茴香与孜然、莳萝子、葛缕子、蛇床子在外观形态上相似,存在掺假及混淆使用的现象。准确鉴别小茴香及其混伪品对维护小茴香产品质量和保护消费者利益具有重要意义。针对传统的形态学和理化鉴别方法的不足,文章通过比较小茴香及其混伪品的叶绿体全基因组,在accD基因中发掘了小茴香可区别于其他混伪品的单核苷酸多态性位点,并分别设计了小茴香及其混伪品的位点特异性引物。在建立的多重位点PCR体系下,小茴香产生了336 bp的特异性条带,其混伪品则产生了168 bp的特异性条带。方法同时验证了小茴香及其混伪品的2个SNP位点,可以在一个反应体系中同时将小茴香与其4种混伪品区分,鉴定结果准确可靠。因此,文章建立的多重PCR体系为小茴香鉴定提供了新的DNA分子标记方法,也为其他香辛料产品来源的鉴定提供了方法借鉴。
Foeniculum vulgare is easy to be confused with Cuminum cyminum,Anethum graveolens,Carum carvi and Cnidium monnieri due to their similarity in appearance.Therefore,accurate identification of F.vulgare and its adulterants is of great significance to ensure the quality of F.vulgare and to protect consumers’ legitimate interests.In order to overcome the deficiency of traditional phenotypic and physiochemical methods,the chloroplast genome of F.vulgare and its adulterants were compared.According to the specific single nucleotide polymorphism (SNP) sites discovered in the accD gene,specific primers were respectively designed for F.vulgare and its adulterants.The developed multiplex allele-specific PCR produced amplicons of 336 bp and 168 bp for F.vulgare and its adulterants,respectively.The method simultaneously confirmed two SNP sites for F.vulgare and its adulterants,and can discriminate F.vulgare from its adulterants in one reaction.Therefore,the established multiplex PCR system provides a new molecular marker method for the identification of F.vulgare,and the method also provides a reference for the origin authentication of other spice products.
作者
王锐莹
于巧宁
王洪涛
申京宇
尹花仙
WANG Ruiying;YU Qiaoning;WANG Hongtao;SHEN Jingyu;YIN Huaxian(College of Life Sicience,Yantai University,Yantai 264005,China)
出处
《食品科技》
CAS
北大核心
2021年第5期260-264,共5页
Food Science and Technology
基金
山东省重点研发计划项目(2018GSF119012)
烟台大学研究生科技创新基金项目(YDYB2119)。
