摘要
【目的】测定蒸汽爆破预处理的杜仲器官经碱煮后溶液中总黄酮的含量,为实现杜仲全值化综合高效利用提供参考。【方法】以杜仲叶片、当年生树皮和种皮为研究对象,采用蒸汽爆破结合催化剂联合预处理工艺,考察稳压时间、汽爆压强、催化剂浓度3个因素对杜仲材料结构变化、比表面积、木质纤维素含量以及干物质损失率的影响;经碱煮处理后,测定溶液中杜仲总黄酮的产率。【结果】在压力为1.0 MPa、稳压时间为120 s的汽爆条件下,叶片的比表面积比对照增加1.24倍,半纤维素和纤维素含量分别比对照降低59.17%和37.44%;经过碱煮后,干物质损失率为80.99%,是对照的2.52倍,溶液中总黄酮含量为164.3810 mg/mL,是对照的4.97倍。在压力为1.8 MPa、稳压时间为220 s的汽爆条件下,树皮的比表面积比对照增加3.42倍,半纤维素和纤维素含量分别比对照降低37.48%和34.97%;经过碱煮后,干物质损失率为61.12%,是对照的1.48倍,总黄酮含量为293.4090 mg/mL,是对照的17.33倍。在压力为2.2 Mpa、稳压时间为220 s的汽爆条件下,种皮的比表面积比对照增加4.80倍,半纤维素和纤维素含量分别比对照降低68.27%和59.19%;经过碱煮后,干物质损失率为55.47%,是对照的1.41倍,总黄酮含量为228.8951 mg/mL,是对照的8.04倍。【结论】蒸汽爆破预处理后,杜仲器官的组织结构被破坏,木质纤维素部分降解,比表面积增大使得溶剂可及性大大提高,碱煮后去除的干物质更多,有利于杜仲器官中总黄酮的提取。
【Objective】In order to comprehensively and efficiently utilize Eucommia ulmoides Oliver,the microstructure and the content of lignocellulose and total flavonoids of E.ulmoides organs pretreated by steam explosion was investigated.【Method】The leaves,one-year-old bark and pericarp of E.ulmoides pretreated by steam explosion combined with catalyst were studied.The effects of three factors(namely the stabilization time,pressure,and catalyst concentration)on the structural change,specific surface area,lignocellulose content and dry matter removal rate of E.ulmoides materials were investigated.The total flavonoids were then measured after alkaline post-treatment.【Result】Under the steam explosion parameters with a pressure of 1.0 MPa and a holding time of 120 s,the specific surface area of the leaves increased by 1.24 times compared to the control.The content of hemicellulose and cellulose decreased by 59.17%and 37.44%compared with the control,respectively.The dry matter was removed by 80.99%after alkaline digestion,which was 2.52 times higher than that of the control.The content of the total flavonoid was 164.3810 mg/mL,which was 4.97 times higher than that of the control.Under a pressure of 1.8 MPa and a holding time of 220 s,the specific surface area of the bark increased by 3.42 times compared with the control,and the content of hemicellulose and cellulose decreased by 37.48%and 34.97%compared with the control respectively.After alkali digestion,the dry matter loss rate was 61.12%,which was 1.48 times more than that of the control.The content of total flavonoid was 293.4090 mg/mL,17.33 times higher than that of the control.The specific surface area of the pericarp increased by 4.80 times compared with the control after steam exploded under 2.2 MPa pressure and 220 s of holding time.The content of hemicellulose and cellulose decreased by 68.27%and 59.19%compared with the control,respectively.The dry matter loss rate was 55.47%after alkaline digestion,which was 1.41 times more than that of the control.The total flavonoid content was 228.8951 mg/mL,which was 8.04 times higher than that of the control.【Conclusion】Steam explosion process destroyed the tissue structure of E.ulmoides organs,leading partially degraded of lignocellulose in cell walls and great increases of the specific surface area,meaning the increases of solvent accessibility.Meanwhile,it helped to increase more dry matter removed.Steam explosion pretreatment was beneficial for the extraction of total flavonoids from E.ulmoides Oliver.
作者
丁欢欢
张宁
王璐
杜庆鑫
杜红岩
刘攀峰
孙志强
DING Huanhuan;ZHANG Ning;WANG Lu;DU Qingxin;DU Hongyan;LIU Panfeng;SUN Zhiqiang(Paulownia R&D Center of National Forestry and Grassland Administration,Zhengzhou 450003,Henan,China;Nanjing Forestry University,Nanjing 210037,Jiangsu,China;Henan Provincial Institute of Science and Technology Information,Zhengzhou 450003,Henan,China;Key Laboratory of Non-timber Forest Germplasm Enhancement&Utilization of State Forestry Administraion,Zhengzhou 450003,Henan,China;Eucommia Engineering Research Center of National Forestry and Grassland Administration,Zhengzhou 450003,Henan,China)
出处
《中南林业科技大学学报》
CAS
CSCD
北大核心
2021年第6期147-156,共10页
Journal of Central South University of Forestry & Technology
基金
中央级公益性科研院所基本科研业务费专项基金项目“蒸汽爆破预处理分离提取杜仲胶技术研究”(CAFYBB2018MB006)
林业科技发展项目“杜仲胶高效提取技术产业化推进与示范”(KJZXZZ2019008)。
关键词
杜仲
蒸汽爆破
结构形态
比表面积
木质纤维素
总黄酮
Eucommia ulmoides Oliver
steam explosion
microstructure
specific surface area
lignocellulose
total flavonoids
