摘要
目的探讨泛素样蛋白D(UBD)在哈萨克族、维吾尔族和汉族3个民族食管鳞癌患者癌及癌旁组织中蛋白和mRNA水平的表达情况,并探究其临床病理学意义。方法收集2011年1月—2014年12月173例食管鳞癌患者癌和癌旁组织标本(距癌组织>5 cm的正常食管黏膜组织),其中包含维吾尔族、哈萨克族和汉族。采用免疫组织化学染色法、实时荧光定量PCR和HE染色法检测UBD的表达水平,并结合患者临床病理特征进行分析。结果3个民族食管鳞癌组织中UBD在蛋白和mRNA水平的表达均高于癌旁组织,差异有统计学意义(P<0.05)。不同民族食管鳞癌组织中,UBD在蛋白和mRNA水平表达量比较,差异无统计学意义(P>0.05)。食管鳞癌组织中UBD表达与神经脉管侵犯、TNM分期、N分期以及分化程度有关(P<0.05),与性别、年龄、T分期、肿瘤部位以肿瘤大小无关(P>0.05)。结论UBD对于食管鳞癌的恶性发展及淋巴结转移可能有重要影响,有望成为预测转移潜能的参考指标。
Objective To investigate the expression of protein and mRNA levels of Ubiquitin D(UBD) with cancer tissues and adjacent tissues in esophageal squamous cell carcinoma patients in Kazakh, Uygur, and Han nationalities, and to explore its clinicopathological significance. Methods A total of 173 cases of esophageal squamous cell carcinoma patients with cancer tissue and adjacent tissue specimens were collected(normal esophageal mucosa tissue> 5 cm from cancer tissue) from 2011 to 2014, including Uyghur, Kazakh and Han nationalities. Immunohistochemical staining method, real-time fluorescent quantitative PCR and HE stai ning method were used to detect the expression level of UBD, and the clinicalpathological characteristics of patients were combined for analysis. Results The protein and m RNA levels of UBD in esophageal squamous cell carcinoma tissues of the three ethnic groups were higher than those of adjacent tissues, and the differences were statistically significant(P < 0.05). In the esophageal squamous cell carcinoma tissues of different ethnic groups, there was no significant difference in the expression of UBD at the protein and mRNA levels(P > 0.05). UBD expression in esophageal squamous cell carcinoma tissues was related to tumor neuro vascular invasion, TNM stage, N stage and differentiation degree(P < 0.05), and it wasn’t related to gender, age, T stage, tumor location, tumor size, etc.(P > 0.05). Conclusion UBD may have an important influence on the malignant development of esophageal squamous cell carcinoma, and it is expected to become a reference indicator for predicting the potential of metastasis.
作者
罗枭磊
卡吾力·居买
孙清超
李德生
买地尼也提·尼亚孜
张力为
伊力亚尔·夏合丁
LUO Xiaolei;KAWULI Jumai;SUN Qingchao;LI Desheng;MADINIYAT Niyaz;ZHANG Liwei;YILIYAER Xiaheding(Department of Thoracic Surgery,the First Affiliated Hospital of Xinjiang Medical University,Xinjiang Uygur Autonomous Region,Urumqi 830054,China;Xinjiang Esophgeal Cancer Research Institute,Xinjiang Uygur Autonomous Region,Urumqi 830054,China;Clinical Medicine Research Institute,the First Affiliated Hospital of Xinjiang Medical University,Xinjiang Uygur Autonomous Region,Urumqi 830054,China)
出处
《中国医药导报》
CAS
2021年第17期13-17,共5页
China Medical Herald
基金
国家重点研发计划精准医学研究专项项目(2017YFC0909900)。
关键词
泛素样蛋白D
食管鳞癌
免疫组织化学
泛素化
Ubiquitin D
Esophageal squamous cell carcinoma
Immunochemistry
Ubiquitination
