摘要
研究新疆北部乌尔禾地区盐渍土壤中微生物群落结构及多样性,以期发现新的高盐环境耐盐性微生物资源菌株。采用传统分离培养法获得可培养耐盐菌株并对菌株形态学、16S rRNA基因测序、耐盐特性进行研究,同时结合高通量测序技术分析新疆乌尔禾地区盐渍土壤耐盐细菌的多样性与群落结构。共分离得到耐盐细菌11株,分属6个属,均为中度耐盐菌,以芽胞杆菌属(Bacillus)为优势菌。对盐渍土壤微生物16S rRNA(V3~V4)基因测序,共获得细菌序列290 952条,分属24个门410个属,变形菌门(Proteobacteria, 60.31%)、厚壁菌门(Firmicutes, 21.52%)、拟杆菌门(Bacteroidetes, 6.9%)和放线菌门(Actinobacteria, 6%)相对丰度较高。优势属为克吕沃尔菌属(Kluyvera,21%)、Hafnia-Obesumbacterium(19.6%)和假单胞菌属(Pseudomonas,7.5%)。结果表明,新疆乌尔禾地区盐渍土壤耐盐细菌优势菌群以芽胞杆菌属(Bacillus)居多,细菌群落结构较复杂,潜在可利用微生物资源较为丰富,对高盐极端环境耐盐微生物新资源有进一步研究的意义。
Microbial community structure and diversity in salinized soil in Wuerhe area of northern Xinjiang were studied, so as to discover new strains of salt-tolerant microbial resources in high-salt environment. Culturable salt-tolerant strains were obtained adopting traditional isolation and culture methods, morphological characteristics, 16 S rRNA gene sequencing, salt tolerance features, high-throughput sequencing technology were simultaneously employed to analyze the diversity and community structure of salt-tolerant bacteria in Wuerhe area in Xinjiang. The results showed that 11 strains isolated belonged to 6 genera and 11 species, all moderate salt-tolerant strains, with the genus of Bacillus as the dominant ones. On the other hand, a total of 290 952 sequences bands of salinized microbial 16 S rRNA(V3-V4) gene sequencing were obtained, they respectively belonged to 24 phyla and 410 genera. Among them Proteobacteria(60.31%), Firmicutes(21.52%), Bacteroidetes(6.9%), and Actinobacteria(6%) with relatively high abundance. The dominant genera were Kluyvera(21%), Hafnia-Obesumbacterium(19.6%), and Pseudomonas(7.5%). Therefore, the dominant bacterial community in salinized soil in Wuerhe area in Xinjiang Bacillus is in the majority, with complex community structure, and rich with potential available microbial resources. Therefore, it is of great significance for further study on new resources of salt-tolerant microorganisms in high-salt extreme environment.
作者
王改萍
阿依古丽·托乎提
王茹
祝长青
WANG Gai-ping;AYIGULI·Tuohuti;WANG Ru;ZHU Chang-qing(Xinjiang Key Lab.of Spec.Species Conserv′n&Reg.Biol.,Coll.of Life Sci.,Xinjiang Normal Uni.,Urumqi 830054)
出处
《微生物学杂志》
CAS
CSCD
2021年第2期17-26,共10页
Journal of Microbiology
基金
国家自然科学基金项目(30960047)
新疆维吾尔自治区教育厅重点实验室“新疆特殊环境物种多样性应用与调控重点实验室”项目(3010010118)
新疆师范大学硕士研究生科研创新项目(XSY201902012)。
关键词
盐渍土壤
耐盐细菌
可培养
群落结构
高通量测序
salinized soil
salt tolerant bacteria
culturable
community structure
high throughput sequencing
